SEE EXAMPLE IN THE IMAGE A mixture of 0.20M acetic acid and 0.30M sodium acetate is given. Calculate the pH of the medium if the pKa of the acetic acid is 4.76. Show your complete solution.
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- Benzalkonium chloride (E=0.16) 1.5g qs 100 ml Distilled water 0.9% Nacl solution ad Make isotonic solution Questions: 1. How many ml of distilled water must be added to make the Benzalkonium chloride preparation isotonic? 2. How many ml of 0.9% NaCl must be added to make a 100ml solution?pH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)at 257 nm) is dissolved in 2.5 mL of buffer. A 250 microliter aliquot is removed and placed in a 1.0 cm A sample of adenosine triphosphate (ATP) (MW 507, ɛ = 14,700 M'cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 2.0. Calculate the weight of ATP in the original 2.5 mL sample. (Choose the correct value from the drop-down list provided. The values of weight are listed in units of milligrams.) Weight of ATP: v mg
- Table 4- Determination of the optimum pH of catechol oxidase enzyme Potato extract (mL) Tube # 2d 3d 4d 5d 6d 7d dH₂O (mL) 0 0 0 0 0 0 Catechol (mL) 6 6 6 6 6 6 pH buffer added 4mL pH 2 4mL pH 4 4mL pH 6 4mL pH 7 4mL pH 8 4mL pH 10 1 1 1 1 1 1 Absorbance (0 mins) A: 0.010 Start Time: 3:24 A: 0.008 Start Time: 3:34 A: 0.023 Start Time: 3:36 A: -0.006 Start Time: 3:36 0.018 A: Start Time: 3:36 A: 0.011 Start Time: 3:36 Absorbance (after 10 mins in 40°C water bath) -0.030 A: Take reading at: 3:44 A: 0.154 Take reading at: 3:46 A: 0-105 Take reading at: 3:46 A: 0-132 Take reading at: 3:46 A: 0.074 Take reading at: 3:46 A: 0.018 Take reading at: 3!46 Q15) What is the enzyme's optimum pH(s)? Answer: Q16) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum pH. Subtract Omin from 10min absorbance -0.04 0.0012 0.082 0.138 0.056 0.007Compute for the lactic acid content of these samples given the values below. Show your solution Volume of 0.1 N NaOH in 1st Sampling: 10 mL Volume of 0.1 N NaOH in 2nd Sampling: 40 mL Volume of sample used: 10mlCalculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.
- Alligation Alternate. Show your complete solution. Note: Indicate the unit. Indicate your answer in whole numbers. 1. The solvent for the extraction of a vegetable drug is 75% alcohol. How many milliliters may 95%, 60% and 50% alcohol be mixed to prepare 75% of 4 pints of alcohol? Amount of 95% alcohol needed = ? Amount of 60% alcohol needed = ? Amount of 50% alcohol needed = ?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.how to calculate these? can you give me the steps or the equations for that? 2ul St.1 St.2 St.3 St.4 Absorbance 595nm 0.926 0.444 0.421 0.462 0.932 0.412 0.407 0.393 Ç Average Abs Corrected Abs 595nm 595nm 0.929 0.428 0.414 0.4275 0.5615 0.0605 0.0465 0.06 [Protein] (µg/ml) 103.98 11.20 8.61 11.11 Volume (ml) 20 40 8 4 [Total Protein] (ug) 2079.63 448.15 68.89 44.44
- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.2. 3. Perform all the calculations necessary for the volume of stock solution or water, in mL, needed to make the dilute solutions listed below. a. b. C. Make: 200 mL of 30% Ethanol dilute solution Stock Volume needed (mL) 95% Ethanol Reagent water Make: 100mL of .09%NaCL Stock 1% NaCl Reagent water 30% SDS 3 M NaOH Make: 100 mL of a 3% SDS and 0.5 MNaOH dilute solution as follows: Stock Volume needed (mL) Reagent water 200 mL Total 1% Lactose 1% Dextrose Volume needed (mL) 100 mL Total Calculate the grams of chemical needed for each stock listed below. Concentration of Stock to Prepare Volume of Stock Needed 1% Sucrose 100 mL Total 10 mL 20 mL 25 mL Amount of chemical Needed (in grams) Your instructor will have you make ONE of the above solutions-ask your instructor which solution to make.Gel Running Buffer is made and kept at 14X concentration for storage. We will need 1.5 L of this solution at a concentration of 1X to run our gels. How would we make this solution? I am having trouble understanding the steps to this question, if you could please help me understand it that would be greatly appreciated!