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SDS-PAGE results: Parameters Percent Resolving Gel Electrophoretogram Legend: 9% 12% Starting from the left-most lane to the right-most lane: Lane 1: 1:1 Crude Extract to Sample Buffer ratio Lane 2: 1:2 Crude Extract to Sample Buffer ratio Lane 3: 1:3 Crude Extract to Sample Buffer ratio 15% Based on the provided results answer the following: 1. What percent of the resolving gel is most appropriate to be used during electrophoresis? Justify. 2. Will you consider the protein bands in SDS-PAGE to have relatively low molecular weights or have relatively high molecular weights? Explain. 3. Does the elution profile of the crude protein extract match the SDS-PAGE results? Account for any differences or similarities (e.g., no. of peaks vs no. of protein bands, observed molecular weights, etc.).

SDS-PAGE results: Parameters Percent Resolving Gel Electrophoretogram Legend: 9% 12% Starting from the left-most lane to the right-most lane: Lane 1: 1:1 Crude Extract to Sample Buffer ratio Lane 2: 1:2 Crude Extract to Sample Buffer ratio Lane 3: 1:3 Crude Extract to Sample Buffer ratio 15% Based on the provided results answer the following: 1. What percent of the resolving gel is most appropriate to be used during electrophoresis? Justify. 2. Will you consider the protein bands in SDS-PAGE to have relatively low molecular weights or have relatively high molecular weights? Explain. 3. Does the elution profile of the crude protein extract match the SDS-PAGE results? Account for any differences or similarities (e.g., no. of peaks vs no. of protein bands, observed molecular weights, etc.).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Tighnari is doing preliminary protein studies on redcrest, a vibrant crimson fruit found in the hot deserts of Sumeru. He extracted proteins in the given sample using phosphate buffer. The resulting crude protein extract was subjected to gel filtration chromatography and SDS-PAGE. GFC results: - Absorbance Peak A B Figure 1. Elution profiles of the mixture of protein standards (black) and crude protein extract (blue). Table 1. Components of the calibration mixture and their corresponding molecular weight. U خلفة X A Elution Volume, mL Identity B Bovine Serum Albumin B-lactoglobulinn Horse Myoglobin Crude Protein Extract Molecular Weight, Da 66000 36800 17600
Transcribed Image Text:Tighnari is doing preliminary protein studies on redcrest, a vibrant crimson fruit found in the hot deserts of Sumeru. He extracted proteins in the given sample using phosphate buffer. The resulting crude protein extract was subjected to gel filtration chromatography and SDS-PAGE. GFC results: - Absorbance Peak A B Figure 1. Elution profiles of the mixture of protein standards (black) and crude protein extract (blue). Table 1. Components of the calibration mixture and their corresponding molecular weight. U خلفة X A Elution Volume, mL Identity B Bovine Serum Albumin B-lactoglobulinn Horse Myoglobin Crude Protein Extract Molecular Weight, Da 66000 36800 17600
SDS-PAGE results: Parameters Percent Resolving Gel Electrophoretogram Legend: 9% 12% Starting from the left-most lane to the right-most lane: Lane 1: 1:1 Crude Extract to Sample Buffer ratio Lane 2: 1:2 Crude Extract to Sample Buffer ratio Lane 3: 1:3 Crude Extract to Sample Buffer ratio 15% Based on the provided results answer the following: 1. What percent of the resolving gel is most appropriate to be used during electrophoresis? Justify. 2. Will you consider the protein bands in SDS-PAGE to have relatively low molecular weights or have relatively high molecular weights? Explain. 3. Does the elution profile of the crude protein extract match the SDS-PAGE results? Account for any differences or similarities (e.g., no. of peaks vs no. of protein bands, observed molecular weights, etc.).
Transcribed Image Text:SDS-PAGE results: Parameters Percent Resolving Gel Electrophoretogram Legend: 9% 12% Starting from the left-most lane to the right-most lane: Lane 1: 1:1 Crude Extract to Sample Buffer ratio Lane 2: 1:2 Crude Extract to Sample Buffer ratio Lane 3: 1:3 Crude Extract to Sample Buffer ratio 15% Based on the provided results answer the following: 1. What percent of the resolving gel is most appropriate to be used during electrophoresis? Justify. 2. Will you consider the protein bands in SDS-PAGE to have relatively low molecular weights or have relatively high molecular weights? Explain. 3. Does the elution profile of the crude protein extract match the SDS-PAGE results? Account for any differences or similarities (e.g., no. of peaks vs no. of protein bands, observed molecular weights, etc.).
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