Introductory Chemistry: An Active Learning Approach
6th Edition
ISBN:9781305079250
Author:Mark S. Cracolice, Ed Peters
Publisher:Mark S. Cracolice, Ed Peters
Chapter3: Measurement And Chemical Calculations
Section: Chapter Questions
Problem 5E
Related questions
Question
Although there are accurate graphical methods available for determining Vmax and Km of an enzyme-catalyzed reaction, sometimes these parameters can be quickly estimated by examining the values of Vo as [S] increases. Estimate the values of Vmax and Km of the reaction catalyzed by an enzyme in which the following data were obtained:
![[S] (M)
2,5 x 10-6
Vo (μM/min)
28
4 x 10-6
40
1 X 10-5
70
2 x 10-5
95
4 x 10-5
112
1 x 104
128
2 x 10-3
139
1 x 10-2
140
2 x 10-1
140](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F2a202615-c0b2-4e50-a408-66e78ca46218%2Faa4f471a-44e6-4229-ad06-f4edfd413e6e%2Fww9lj29_processed.jpeg&w=3840&q=75)
Transcribed Image Text:[S] (M)
2,5 x 10-6
Vo (μM/min)
28
4 x 10-6
40
1 X 10-5
70
2 x 10-5
95
4 x 10-5
112
1 x 104
128
2 x 10-3
139
1 x 10-2
140
2 x 10-1
140
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