s are purified and sequenced. The sequences of th eaves after lysine (K) and arginine (R); cyanogen br .Work to show the sequence of peptide using eithe Cyanogen bromide C1: TKDCVHSD EPIDPR

General, Organic, and Biological Chemistry
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ISBN:9781285853918
Author:H. Stephen Stoker
Publisher:H. Stephen Stoker
Chapter20: Proteins
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Problem 20.58EP: For the tripeptide SerValMet a. What amino acid is located at the peptides N-terminal end? b. What...
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Q21. A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide,
and the peptides are purified and sequenced. The sequences of the peptides are shown below.
(note: trypsin cleaves after lysine (K) and arginine (R); cyanogen bromide cleaves after
methionine (M)). Work to show the sequence of peptide using either Ts or Cs. (6; 84)
Trypsin peptide
Cyanogen bromide peptides
C1: TKDCVHSD
T1: EGAAYHDFEPIDPR
T2: GASMALIK
C2:
EGAAYHDFEPIDPRGASM
T3: YLIACGPMTK
C3: ALIKYLIACGPM
T4: DCVHSD
Transcribed Image Text:Q21. A polypeptide is cleaved into peptides by treatment with trypsin and cyanogen bromide, and the peptides are purified and sequenced. The sequences of the peptides are shown below. (note: trypsin cleaves after lysine (K) and arginine (R); cyanogen bromide cleaves after methionine (M)). Work to show the sequence of peptide using either Ts or Cs. (6; 84) Trypsin peptide Cyanogen bromide peptides C1: TKDCVHSD T1: EGAAYHDFEPIDPR T2: GASMALIK C2: EGAAYHDFEPIDPRGASM T3: YLIACGPMTK C3: ALIKYLIACGPM T4: DCVHSD
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