Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. Western analysis for BTG2, AKT, p-AKT, ERK1/2 and p-ERK1/2 in MDA-MB-231 cells transfected with 1. inhibitor-control (inhibitor-ctrl) and siBTG2 scrambled oligonucleotide (si-ctrl) 2. miRNA inhibitor and si-ctrl, 3. inhibitor and siBTG2 4. inhibitor-ctrl and siBTG2 Protein expression was quantified by band intensity and normalized to GAPDH. MDA-MB-231 BTG2 AKT p-AKT ERK1/2 p-ERK1/2 GAPDH elative Protein Level 1.51 1.0- 0.5- miR-NC BTG2 PAKT PERK _ miR-inhibitor m NC+siBTG inhibitor+siBTG

Curren'S Math For Meds: Dosages & Sol
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Chapter10: Reconstitution Of Powdered Drugs
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Please help with answering this Cancer Biology question. Detailed answers are welcome to aid in understanding.

Refer to the experimental approach (i.e., very briefly describe) and give the results AND
conclusions. The answer is incomplete without reference to all the data including controls.
Western analysis for BTG2, AKT, p-AKT, ERK1/2 and p-ERK1/2 in MDA-MB-231 cells
transfected with
1. inhibitor-control (inhibitor-ctrl) and siBTG2 scrambled oligonucleotide (si-ctrl)
2. miRNA inhibitor and si-ctrl,
3. inhibitor and siBTG2
4. inhibitor-ctrl and siBTG2
Protein expression was quantified by band intensity and normalized to GAPDH.
MDA-MB-231
BTG2
AKT
p-AKT
ERK1/2
p-ERK1/2
GAPDH
Relative Protein Level
1.51
1.0-
0.5-
110
0.0-
inhibitor-ctrl +
si-ctrl +
inhibitor
siBTG2
miR-NC
BTG2
PAKT
PERK
miR-inhibitor
m
NC+siBTG
inhibitor+siBTG
Transcribed Image Text:Refer to the experimental approach (i.e., very briefly describe) and give the results AND conclusions. The answer is incomplete without reference to all the data including controls. Western analysis for BTG2, AKT, p-AKT, ERK1/2 and p-ERK1/2 in MDA-MB-231 cells transfected with 1. inhibitor-control (inhibitor-ctrl) and siBTG2 scrambled oligonucleotide (si-ctrl) 2. miRNA inhibitor and si-ctrl, 3. inhibitor and siBTG2 4. inhibitor-ctrl and siBTG2 Protein expression was quantified by band intensity and normalized to GAPDH. MDA-MB-231 BTG2 AKT p-AKT ERK1/2 p-ERK1/2 GAPDH Relative Protein Level 1.51 1.0- 0.5- 110 0.0- inhibitor-ctrl + si-ctrl + inhibitor siBTG2 miR-NC BTG2 PAKT PERK miR-inhibitor m NC+siBTG inhibitor+siBTG
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