Reductive power. What ratio of NADPH to NADP+ is required to sustain [GSH] = 10 mM and [GSSG] = 1 mM ? Use the redox potentials given in Table 18.1 . TABLE 18.1 Standard reduction potentials of some reactionsOxidantSuccinate + CO₂2AcetateFerredoxin (oxidized)2 H+NAD+NADP+Lipoate (oxidized)Glutathione (oxidized)FADAcetaldehydePyruvate2 H+FumarateCytochrome b (+3)DehydroascorbateUbiquinone (oxidized)Cytochrome c (+3)Fe (+3)¹/2 O₂ + 2H+Reductanta-KetoglutarateAcetaldehydeFerredoxin (reduced)H₂NADH + H+NADPH + H+Lipoate (reduced)Glutathione (reduced)FADH₂EthanolLactateH₂SuccinateCytochrome b (+2)AscorbateUbiquinone (reduced)Cytochrome c (+2)Fe (+2)H₂On221222222 N N N N N N N22~122112Note: E' is the standard oxidation-reduction potential (pH 7, 25°C) and n is the number of electronstransferred. E' refers to the partial reaction written as Oxidant + e → reductant¹Standard oxidation - reduction potential at pH = 0.E' (V)-0.67-0.60-0.43-0.42-0.32-0.32-0.29-0.23-0.22-0.20-0.19-0.00¹+0.03+0.07+0.08+0.10+0.22+0.77+0.82

Within a cell, reactive oxygen species are produced as a byproduct of oxygen metabolism and the cell…

Answered: Reductive power. What ratio of NADPH to…

Reductive power. What ratio of NADPH to NADP+ is required to sustain [GSH] = 10 mM and [GSSG] = 1 mM ? Use the redox potentials given in Table 18.1

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Reductive power. What ratio of NADPH to NADP+ is required to sustain [GSH] = 10 mM and [GSSG] = 1 mM ? Use the redox potentials given in Table 18.1 .

TABLE 18.1 Standard reduction potentials of some reactions
Oxidant
Succinate + CO₂2
Acetate
Ferredoxin (oxidized)
2 H+
NAD+
NADP+
Lipoate (oxidized)
Glutathione (oxidized)
FAD
Acetaldehyde
Pyruvate
2 H+
Fumarate
Cytochrome b (+3)
Dehydroascorbate
Ubiquinone (oxidized)
Cytochrome c (+3)
Fe (+3)
¹/2 O₂ + 2H+
Reductant
a-Ketoglutarate
Acetaldehyde
Ferredoxin (reduced)
H₂
NADH + H+
NADPH + H+
Lipoate (reduced)
Glutathione (reduced)
FADH₂
Ethanol
Lactate
H₂
Succinate
Cytochrome b (+2)
Ascorbate
Ubiquinone (reduced)
Cytochrome c (+2)
Fe (+2)
H₂O
n
2
2
1
2
2
2
222 N N N N N N N
2
2
~12
2
1
1
2
Note: E' is the standard oxidation-reduction potential (pH 7, 25°C) and n is the number of electrons
transferred. E' refers to the partial reaction written as Oxidant + e → reductant
¹Standard oxidation - reduction potential at pH = 0.
E' (V)
-0.67
-0.60
-0.43
-0.42
-0.32
-0.32
-0.29
-0.23
-0.22
-0.20
-0.19
-0.00¹
+0.03
+0.07
+0.08
+0.10
+0.22
+0.77
+0.82

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