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- Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…m BIO101 Short EXAM First session X G Google om/moodle/mod/quiz/attempt.php?attempt%3D1313727&cmid%3D298536 e on the bookmarks bar. Import bookmarks now... AL BIOLOGY I/ ill courses GENERAL BIOLOGY I BIO101 Short EXAM First sess Time left 0:21:42 If an enzyme in solution is saturated with its substrate, the most effective way to obtain a higher reaction rate is to: O a. add more substrate O b. heat the solution to 95°C O c. add more of the enzyme O d. All of the options are correct O e. add a noncompetitive inhibitor Next page vity Jump to.. ents 51°F Light rairSelect true if the statement is CORRECT and false if OTHERWISE 1. Enzymes are catalysts and increase the speed of a chemical reaction without themselves undergoing any permanent chemical change. 2. Catalysis is defined as the acceleration of a chemical reaction 3. if the amount of the enzyme is kept constant and the substrate concentration is then gradually increased, the reaction velocity will decrease. 4. In the Induced-fit Model, if a dissimilar substance which does not fit the site is present, the enzyme rejects it 5. The Michaelis constant Vo is defined as the substrate concentration at 1/2 the maximum velocity. 6. A prosthetic group - an organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion. 7. The rate of an enzyme-catalyzed reaction increases as the temperature is raised beyond optimum temperature. 8. Enzymes can be classified by the kind of chemical reaction catalyzed. 9. The living cell is the site of tremendous…
- D 1. 0.25 Short answer questions, write answers on blank paper. A plot of enzyme activity with and without an inhibitor present gave the following plot. 0.2 0.15 1/V v in mM/s 0.1 0.05 0 0 0.05 0.1 y=0.67x+0.02 0.15 y=0.2x+0.02 0.2 0.25 0.3 1/[S]. [S] in mM Without the inhibitor, what are the values of Vmax and Km? In the presence of the inhibitor, which of these items has changed and what is (are) the new value(s)? What type of inhibitor is present (explain why you have arrived at this conclusion)? Be sure to include units as necessary--answers that do not contain required units will be marked incorrectCase Study: Case Study: Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30'C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of охудen bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a…True or false? Initial velocity studies are particularly useful for investigating the kinetics of an enzyme catalyzed reaction? Substantiate your answer
- a. What is more? Activity 4 "Fill Me In!" Directions. Analyze the chemical equation. Supply the missing word to complete the paragraph. Write your answer on the space provided. 6CO2 + 6 H20 + energy CeH1206 + 602 The process shown by this chemical equation is (1) This equation shows that when (2) water to carbon dioxide, (3) oxidized and (5) atoms are transferred from is formed. (4) has been has been reduced. C6H1206 + 6O2 6 CO2 + 6 H20 energy The process shown in this chemical equation is (6) In this reaction glucose has been (7)_ and oxygen has been (8) since it has lost its hydrogen atoms since it has gained hydrogen atoms. MY SCORE: a. What is more? Activity 4 "Schema Creation" Directions. Consider the graph below to answer the questions inside the box. Write your answer on the space provided. 1. What is the temperature that promotes highest percentage of enzymatic activity? 90 IA 80 70 2. At what point does the enzyme completely decrease its activity? 60 50 40 30 3. Why does the…me Lab Evidence-Bas X NC Grades for Beatrice Pierre: 2024 X ourses/192624/quizzes/1488062/take Question 1 Background: While completing the enzyme lab exercises, you learned about the enzyme called catalase, which catalyzes the decomposition of H₂O2 hydrogen peroxide to O₂ (oxygen) and H₂O (water). You have performed experiments to investigate the effect of the following three conditions Substrate concentration, temperature, and pH. During the lab, you generated data, graphed your results, and made general statements about your results. Drawing Evidence-Based Conclusions: Write an overall evidenced-based conclusion about catalase that answers the question below. Your response should include supporting evidence from all of your data (3-4 sentences). Edit View Insert Format Tools Table B I U b My Questions | bartleby What effect did substrate concentration, temperature, and pH have on the activity of catalase? 12pt 3 pts Q Sea Paragraph xThe body uses ATP for several activities such as Muscle contraction, transport of molecules etc. Reflect on the main metabolic processes or pathways involved in ATP formation from each of the various macro molecules.Indicate the processes or pathways involved with ATP formation with each macro-molecule respectively.
- Conversion Efficiency definition?252F%252Fbbniagaraccc.sin.suny.edu%252Fwebapps signment Saved He One of the uses of diazepam is as an antiepileptic that can be administered by slow IV infusion at a rate of 0.5 mg/min for 1 hour. If the infusion solution consists of 6 ml and there are 15 drops per milliliter of solution, how many drops per minute should be administered? drops per minuteOCPS Das X ← → CO PS Login OCPS + Orders - X Performance Matters Question 12 of 16 Rate of reaction Previous Performa X olaocps.performancematters.com/ola/ola.jsp?clientcode=flocps# Join a Game - Quizizz K! Play Kahoot! - Enter... V C Educator X 19 Exam: 01 × Plans for X Submit Test Figure 1. Enzyme Rate of Reaction X = point of saturation The diagram below shows the relationship between substrate concentration and reaction rate. Dashboard ↑ Increasing concentration does not affect reaction rate AP EL U.S. Citize X F https://sex Performance Matte... S FLVS Login Welcome, Asheley! 02.Cl.Biology.CRM3.2_2023 4JypvK8X X + OT QMy Quizzes - Quizizz Athletic Clearance Substrate concentration Based on the graph, which of the following describes why continually increasing substrate does not lead to a continuous increase in reaction rate?