Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Question 2. You want your recombinant Human Growth Hormone to be tagged at the N-terminus with a poly- histidine domain (which is present in the vector), so you need your to cloned in a way that maintains the reading frame. To facilitate cloning, you have added an EcoR1 restriction site to the N-terminus of the Human growth hormone, as an EcoR1 site is also present in the vector (EcoR1 sites in red; N-terminus of HGH in blue). Vector 1: Vector 2: Vector 3: i) Answer: ii) His His His His His His Gly Asn Ser Gly Gln CAT CAT CAT CAT CAT CAT GGG AAT TCG GGC CAA DNA to be inserted into the vector: Answer: His His His His His His Glu Phe Gly Pro Lys CAT CAT CAT CAT CAT CAT GAA TTC GGG CCC AAA His His His His His His Gly Ile Arg Ala Gln CAT CAT CAT CAT CAT CAT GGA ATT CGG GCC CAA Which vector will you use, 1, 2, or 3? Met Ala Thr Gly... AACAAGAATTCTATG GCT ACA GGG... Draw the final DNA (bottom) and tagged protein (top) sequences after you have ligated the insert to the proper vector

Question 2. You want your recombinant Human Growth Hormone to be tagged at the N-terminus with a poly- histidine domain (which is present in the vector), so you need your to cloned in a way that maintains the reading frame. To facilitate cloning, you have added an EcoR1 restriction site to the N-terminus of the Human growth hormone, as an EcoR1 site is also present in the vector (EcoR1 sites in red; N-terminus of HGH in blue). Vector 1: Vector 2: Vector 3: i) Answer: ii) His His His His His His Gly Asn Ser Gly Gln CAT CAT CAT CAT CAT CAT GGG AAT TCG GGC CAA DNA to be inserted into the vector: Answer: His His His His His His Glu Phe Gly Pro Lys CAT CAT CAT CAT CAT CAT GAA TTC GGG CCC AAA His His His His His His Gly Ile Arg Ala Gln CAT CAT CAT CAT CAT CAT GGA ATT CGG GCC CAA Which vector will you use, 1, 2, or 3? Met Ala Thr Gly... AACAAGAATTCTATG GCT ACA GGG... Draw the final DNA (bottom) and tagged protein (top) sequences after you have ligated the insert to the proper vector

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
Please send me the question in 20 minutes it's very urgent plz
Question 2. You want your recombinant Human Growth Hormone to be tagged at the N-terminus with a poly- histidine domain (which is present in the vector), so you need your to cloned in a way that maintains the reading frame. To facilitate cloning, you have added an EcoR1 restriction site to the N-terminus of the Human growth hormone, as an EcoR1 site is also present in the vector (EcoR1 sites in red; N-terminus of HGH in blue). Vector 1: Vector 2: Vector 3: i) Answer: ii) His His His His His His Gly Asn Ser Gly Gln CAT CAT CAT CAT CAT CAT GGG AAT TCG GGC CAA DNA to be inserted into the vector: Answer: His His His His His His Glu Phe Gly Pro Lys CAT CAT CAT CAT CAT CAT GAA TTC GGG CCC AAA His His His His His His Gly Ile Arg Ala Gln CAT CAT CAT CAT CAT CAT GGA ATT CGG GCC CAA Which vector will you use, 1, 2, or 3? Met Ala Thr Gly... AACAAGAATTCTATG GCT ACA GGG... Draw the final DNA (bottom) and tagged protein (top) sequences after you have ligated the insert to the proper vector
Transcribed Image Text:Question 2. You want your recombinant Human Growth Hormone to be tagged at the N-terminus with a poly- histidine domain (which is present in the vector), so you need your to cloned in a way that maintains the reading frame. To facilitate cloning, you have added an EcoR1 restriction site to the N-terminus of the Human growth hormone, as an EcoR1 site is also present in the vector (EcoR1 sites in red; N-terminus of HGH in blue). Vector 1: Vector 2: Vector 3: i) Answer: ii) His His His His His His Gly Asn Ser Gly Gln CAT CAT CAT CAT CAT CAT GGG AAT TCG GGC CAA DNA to be inserted into the vector: Answer: His His His His His His Glu Phe Gly Pro Lys CAT CAT CAT CAT CAT CAT GAA TTC GGG CCC AAA His His His His His His Gly Ile Arg Ala Gln CAT CAT CAT CAT CAT CAT GGA ATT CGG GCC CAA Which vector will you use, 1, 2, or 3? Met Ala Thr Gly... AACAAGAATTCTATG GCT ACA GGG... Draw the final DNA (bottom) and tagged protein (top) sequences after you have ligated the insert to the proper vector
Expert Solution
steps

Step by step

Solved in 3 steps with 2 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS