Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution Final [NaCI] (mM) number 1 150 120 90 4 60 30 6.
Protocol: You are provided with a suspension of (outdated) human RBCS and 1M NaCl solution. Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube (1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown in Table 1 below. Ensure each of your tubes are properly labelled for future identification. Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl concentrations shown in the last column of Table 1 below: Table 1 Vol. of stock 1M NaCl to add (µL) Vol. of water to add (uL) Tube/solution Final [NaCI] (mM) number 1 150 120 90 4 60 30 6.
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter6: Solutions And Colloids
Section: Chapter Questions
Problem 6.89P
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Solve the question with calculations
Table and protocol to be referred to
![PART A
Protocol: You are provided with a suspension of (outdated) human RBCS and 1M
NaCl solution.
Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube
(1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown
in Table 1 below.
Ensure each of your tubes are properly labelled for future identification.
Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl
concentrations shown in the last column of Table 1 below:
Table 1
Final [NaCl] (mM)
Vol. of stock 1M
NaCl to add (µL)
Vol. of water to
add (uL)
Tube/solution
number
1
150
2
120
90
4
60
30
6](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1465368f-24d2-4ff7-8875-eb5112b4d722%2F141bb8be-8e44-4ef7-9580-4761546f4cbf%2Faxuz9qp_processed.png&w=3840&q=75)
Transcribed Image Text:PART A
Protocol: You are provided with a suspension of (outdated) human RBCS and 1M
NaCl solution.
Calculate the volumes (in uL) of water and 1M NaCl you need to add to each tube
(1-6) to yield a final volume of 1 mL at the final NaCl concentrations (in mM) shown
in Table 1 below.
Ensure each of your tubes are properly labelled for future identification.
Make additions to 6 microcentrifuge (Eppendorf) tubes as calculated to yield NaCl
concentrations shown in the last column of Table 1 below:
Table 1
Final [NaCl] (mM)
Vol. of stock 1M
NaCl to add (µL)
Vol. of water to
add (uL)
Tube/solution
number
1
150
2
120
90
4
60
30
6
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