Project 2: Food Dye Spectroscopy How can you use serial dilutions to determine the relationship between concentration and absorbance? Graphs may also help in this explanation.
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Project 2: Food Dye Spectroscopy
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- How will you use a solution of known concentration of one of the food dyes to determine the relationship between concentration and absorbance? Be sure to explain how you determined the wavelength for your analysis.5. A sample of a red dye and a sample of a blue dye both have the same concentration. Their Absorbance is measured using the same spectrophotometer. At a particular wavelength, will both samples give the same Absorbance reading? Why or why not?Which of the following explains why a more concentrated dye solution has a higher absorbance? A. There are more dye molecules in the solution so more isotopes reach the detector. b. There are more isotopes in solution to emit photons. c. There are more dye molecules in solution to emit isotopes. d. There are more isotopes in solution to emit protons. e. There are more dye molecules in the solution to absorb photons.
- 2. Calculate the absorbance value (A) for a solution that absorbs 89.5% of the light striking it. (Hint: What is the percent transmittance? Review the background reading)Project 2: Food Dye Spectroscopy What is the relationship between absorbance and transmittance?Food dye spectroscopy 2. At what wavelengths should the readings be taken? What instrumentation will you utilize with a solution of a known dye to determine the wavelength for your analysis?
- True or False 1. Photodiode arrays are devices within the instrument that help amplify the signal of a sample. 2. Atomic spectroscopy requires the atomization of a sample, which involves nebulization and eventually volatilization to form an elemental vapor. 3. Organic compounds are normally analyzed in atomic spectroscopy despite their small electronic transition range.Which is TRUE for spectroscopy of a mixture? A. The absorbance for a mixture at a particular wavelength is the sum of the absorbances for the components that absorb at the particular wavelength. B. Spectrophotometers can differentiate between mixture components that absorb at the same wavelength. C. Each component in a mixture has the same molar absorptivity at the same wavelength. D. The concentration for each component in a mixture is easily calculated by least squares for guesses of each component when the individual spectra are well resolved.Help interpreting IR.
- A. dependent variable independent variable B) Graph B C) Graph C D) Graph D E) Graph E D. In 700 wavelength (nm) 400 B. of 700 400 wavelength E. Which graph above best illustrates the relationship between the concentration of a substance and the height of its absorbance peak? A) Graph A C. time time3. You have two solutions containing the same compound having the same pink color. The concentration of the first solution is 2.0 M, while the concentration of the other solution is 2.5 M. Absorbance of each solution was measured with the same spectrophotometer at the same wavelength. a) Which solution has the more intense pink color? b) Which solution has the higher absorbance?Do your graphs show the trends a)you expected based on what you know about absorbance and transmittance using a spectrophotometer? Why or why not? b) why does absorbance data relate more conveniently to concentration that does transmission data?