Procedure Take ml Top up to 50ml. Add 50 ul of Mix Sml overnight culture mixture analyse to Add fresh LB ampicillin to the the mixture with 45 ml LB both broth OD600 Withdraw 3ml mixture to Measure every 15 mins. Stop until" 75 mins. The mixture is then - incubated incubator at 37°c 200rpm Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) Mix overnight culture Sml Take 3 ml mixture No ampicillin to No ampicilin B (control) with 45 ml LB broth analyse OD600 the Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to - analyse (OD600)record as zero time-point. at shaked incubator at 37°c 200rpm Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) - Group 2 (Ampicilin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)
Procedure Take ml Top up to 50ml. Add 50 ul of Mix Sml overnight culture mixture analyse to Add fresh LB ampicillin to the the mixture with 45 ml LB both broth OD600 Withdraw 3ml mixture to Measure every 15 mins. Stop until" 75 mins. The mixture is then - incubated incubator at 37°c 200rpm Overnight culture -analyse (OD600)record as zero time-point. at shaked (E-coli + LB borth) Mix overnight culture Sml Take 3 ml mixture No ampicillin to No ampicilin B (control) with 45 ml LB broth analyse OD600 the Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to - analyse (OD600)record as zero time-point. at shaked incubator at 37°c 200rpm Graph of Absorbance vs Time (mins) 18 16 14 1.2 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) - Group 2 (Ampicilin) Group 2(Control) Measurement of bacterial cell growth in LB Absorbance(60onm)
Chapter14: Formula Method
Section: Chapter Questions
Problem 5SST
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