potnesis for transcription of minus-sense ssRNA viruses was best supported by: O The effect of alpha-amanatin on transcription of the genome O The ability to replicate in human and insect cells O The presence of small sub-genomic antisense RNAS O The UV-target sensitivity of transcripts is greater than their coding size the further from the 3-end you go
Q: Explain the mechanism by which some (+)ssRNA viruses are able to produce many (e.g. ~10) proteins…
A: In general viruses depend on host cells for: (1) energy, mainly in the form of nucleoside…
Q: What is the significance of negative strand synthesis of positive a ssRNA virus? a. Loss of…
A: Viruses can have DNA or RNA as genetic material. A positive-strand single-stranded RNA virus has the…
Q: The palm domain of poliovirus RdRP contains a GDD motif. Explain why changing D (Asp) in the GDD to…
A: Though both aspartate and glutamate amino acids are negatively charged but there is a little…
Q: rk band in the relevant western blot image within the figure. Drug X Drug Y UT Ligand Ligand Ligand…
A: Introduction Phosphorylation is the addition of phosphate group.
Q: What if you designed a CRISPR/cas9 assay for a gene knockout, but there's no PAM sequence…
A:
Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…
A: Base editing It is an alternative tool to HDR-mediated replacement. This facilitates editing of…
Q: he bands indicated by arrow "A" (to the left of the western blot) most likely represent: A.…
A: The bands indicated by arrow "A" (to the left of the western blot) most likely represent:-
Q: Although many +ssRNA viruses use their genomr directly as mRNA retroviruses do not. instead their…
A: Retroviruses have a very unique mechanism . They are single stranded RNA viruses, instead of using…
Q: Compare and contrast the roles of crRNA and tracrRNA in the defense process against bacteriophages…
A: Cas9 in conjunction with the engineered CRISPR sequences can be used to hunt down the codes and them…
Q: 1- which of the following can be used for treating/combating bacterial infections a. targeting…
A: Bacteria are unicellular organisms, having sizes of 0.5 micrometers to 2 micrometers. They cause…
Q: In this western blot, the levels of TBK remain constant with increasing amounts/expression of the…
A: TBK-1 gene encodes an enzyme known as TBK-1 (TANK binding kinase-1). This is a…
Q: One of the causes of morbidity for the Zika Virus is Guillain-Barré Syndrome, an immune attack of…
A: Zika virus has family - flaviviridae. This virus spreds with the help of aedes mosquitoes. They…
Q: 15. The CRISPR/Cas9 system provides a form of adaptive immunity in bacteria. We discussed in class…
A: The correct option is DThe CRISPR/Cas9 system is a bacterial immune system to provide protection…
Q: Directions: This is a manual simulation of COVID-19 RT-PCR test. Determine if the given results of…
A: The synthesis of m RNA is from the template strand of DNA which is complementary to the coding…
Q: In this western blot, the levels of phosphorylated TBK (PTBK) decrease with increasing…
A: Western blot analysis is the assay done to check expression of protein. In this method, proteins are…
Q: Explain the Gene Expression Experiment Re-Analysis Summary of Rabies Virus(Taxon:11292) Explain how…
A: Rabies is a viral disease that can be prevented by avoiding contact or bite from a rabid animal.…
Q: The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the…
A: A protospacer adjacent motif (PAM) is a 2–6 base pair DNA sequence tareted by Cas9 nuclease.
Q: The image at right shows (A) RNA blot analysis (hsp1) and (B) protein abundance (HSP, Actin) for…
A: Heat shock proteins Heat shock proteins are the molecular chaperones that help in refolding of…
Q: You are assigned to create new MRNA vaccine. You identified target protein (gene) for vaccine and…
A: An mRNA vaccine uses a type of mRNA to induce an immune response in the body example COVID vaccine…
Q: An enzyme is known to transport lactose into the cell. Answer: Mutations in CFTR gene can cause O a.…
A: Introduction: Enzymes are proteins that serve as biocatalysts. They enhance the rate of reactions…
Q: The clustered, regularly interspaced short palindromic repeats (CRISPR) system is like a bacterial…
A:
Q: 1. Hemagglutination and hemagglutination inhibition assays can be used for the following purposes,…
A: Hemagglutination and hemagglutination inhibition assays - Hemagglutination - It is a kind of…
Q: plain the fundamental difference between CRISPR/Cas9 and in vivo adenovirus-based gene therapy
A: All biological data of AN organism is held on within the biomolecule known as DNA. gene is the…
Q: Consider a bacterial promoter with -35 and -10 elements. What assay is best to show that RNA…
A: The electrophoretic mobility shift assay (EMSA) is a method for the detection of protein-nucleic…
Q: 8. Of the following molecular events, which is the most critical for phenotypic expression? a. the…
A: Introduction :- Molecular biology involves many kind of molecular events that takes place inside the…
Q: Before the integration of a transposon, its transposasemakes a staggered cut in the host target DNA.…
A: Introduction Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil…
Q: 1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3. Name one…
A: Hi! Thanks for your question. As the second part of the question (b) is incomplete because it does…
Q: What is the actual biological purpose of the CRISPR/Cas9 system?
A: All biological information of an organism is stored inside the biomolecule called DNA. Gene are the…
Q: Look at the structures and predict the type of inhibition (i.e. competitive or non-competitive)…
A: Competitive inhibitor is a structural analogue of the substrate and competes for active site of…
Q: which step of CRISPR system the Cas 9 enzyme causes the double strand break D a. Type III CRRNA…
A: Crispr stands for clusteted regularly interspersed short palindromic sequence. CRISPR “spacer”…
Q: We are close to having 2 RNA vaccines for covid. (8 pts) Use what we know about the chemical…
A: mRNA vaccine is the type of immunization technique which uses central dogma of molecular biology to…
Q: Bacteriophages can recognize the host cell by: O a. Binding of a viral protein to a receptor on the…
A: Note: We’ll answer the first question since the exact one wasn’t specified. Please submit a new…
Q: 2. You are planning to clone a gene into the PBR322 plasmid vector. The gene has Bgl II restriction…
A: Gene cloning is the technique to produce the exact copies of the desired gene by utilizing genetic…
Q: Cas9 endonuclease can be targeted to specific sequences in the genome using a OA. PAM site O B.…
A: Answer:- Option B is the correct choice. Cas9 endonuclease is a bacterial RNA-guided substance It…
Q: Reverse transcriptases do not proofread as they synthesize DNA using an RNA template. What do you…
A: Introduction A reverse transcriptase is an enzyme that produces complementary DNA from an RNA…
Q: The COVID-19 vaccines are RNA-based (the RNA codes for a viral antigen). Nucleic acid-based…
A: The vaccine is a substance that activates a person's immune system to develop immunity to a…
Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…
A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…
Q: Describe any three vectors less method of introducing the rDNA into a competent host cell?
A: rDNA is the recombinant DNA which is formed by combining desired genes from different organisms. It…
Q: For transcription of middle promoters in T4 phage, the anti-sigma factor, AsiA, will а. bind -10…
A: The bacteriophage T4 Alt gene product is a component of the phage head and, together with the phage…
Q: he lower limit of detection of an EBV assay is 25 copies/mL. All controls were as expected, and the…
A: EBV assay is a common tool to measure the presence of the virus in clinical samples. The lower limit…
Q: Need help, please.
A: Introduction DNA cloning or a gene cloning is a classical method in which artificial vectors are…
Q: Predict the replication step for the novel Miovirus. This virus was determined to have a dsRNA…
A: Introduction A virus is a small piece of genetic material, such as DNA or RNA, encased in a protein…
Q: Describe the biosynthesis Filovirus (Marburg virus genome. Discuss the transcription of the virus…
A: *Filovirus cause hemorrhage in humans. *Filovirus genome has negative sense RNA virus. *Negative…
Q: formation of LTRs?
A: LTR RETROTRANSPOSON The genomic RNA is packaged in the virion with the retroviruses specific…
Q: Gene therapy can be done by? O Lamda phage totipotent O CRISPR-Cas9 O pluripotent O dihybrid
A: Gene therapy is a technique which modifies a persons genes to treat diseases. There are two types of…
Q: In this western blot, the levels of actin increase with increasing amounts/lexpression of the viral…
A: Introduction :- Western blot is an technique , similar as that of southern( used to detect DNA)…
Q: The new Covid19 vaccine produced by two companies (Pfizer, Moderna) uses mRNA coding for part of the…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: A DNA fragment of interest is inserted in the BamHI site of plasmid pBR322.The recombinants will be:…
A: Genetic Recombination: Recombination is a process of alteration of the existing genetic properties…
Q: RNAi: a) is a cellular defense system against viral replication b) involves dicer…
A: RNA stands for ribonucleic acid. It consists of ribonucleotide sub units.
Step by step
Solved in 2 steps
- The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future. Gene therapy for adenosine deaminase deficiency has proved to be quite promising, but many obstacles remain to be overcome. Currently, the correction of human genetic defects is done using retroviruses as vectors. For this purpose, viral genes are removed from the retroviral genome, creating a vector capable of transferring human structural genes into sites on human chromosomes within target-tissue cells. Do you see any potential problems with inserting pieces of a retroviral genome into humans? If so, are there ways to combat or prevent these problems?5a The two factors that bind directly to the DNA at Pacific sequences are TFIID and TFIIB- in general terms what is the function of each of these two transcription factors in the initiation of transcription This is not a graded question and it will never be graded, This is for studying purpose onlyQuestion:- There is some recent research on the use of bacteriophages to inhibit pathogenic bacteria as alternative to antibiotics, for example, in poultry. Speculate what are the potential advantages and weaknesses of such phage therapy vs standard antibiotic treatment.
- 08 Nucleoside and nucleotide analogs are two closely related classes of anti-viral drugs that inhibit viral DNA or RNA polymerase enzymes. What is their most common mechanism of action and why are they often referred to as "chain terminators"? Edit View Insert Format Tools Table A xd0 Paragraph v They are referred as chain terminators because the sturcutre when looked at does not contain a 3 prime end 31 8 8:10 EE O 6 nQUESTION: Transcribe the gene. Write out the correct sequence of m RNA bases. Notice that this is the same gene as in B above. Recall that in RNA, thymine (T) does not exist and uracil (U) takes its place. 3' ССА TAG CÁC CTT GTC ACA ACG TGT TCG TAG ACA 1 3. 4. 6. 7. 8. 9- 10 11 AGG AAC АТА ATA GTT AAC CTT TTG ATA ACA ТТА ACT 12 13 14 15 16 17 18 19 20 | 21 inChoose correct option and do explain plz. 1. The protein complex that helps RNA polymerase to cross nucleosomes during extension is:a. SWI-SNFb. poly A polymerasac. TFIISd. FACT 2. The polydenylation is carried out by:a. primaseb. polymers poly ac. a reverse transcriptased. adenylyltransferase 3. The lactose operon produces a polycistronic mRNA that includes four genes: Lacl, LacZ, LacY and LacA. True or false?
- Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.QUESTION 2 If you made a change in the DNA of a promoter sequence that inactivates the promoter, what would happen at the RNA level? O The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made The mutation of the DNA would be carried through to the RNA sequence The RNA polymerase would not be able to recognize and bind the DNA, so no RNA would be made Nothing, because the RNA would be made as usualTATAAAA A > > +1 B Start codon 5'-UTR 3'-UTR ↑ D Polyadenylation signal ATG T E Transcription start site Stop codon Ribosome binding site Binding site of the transcription initiation complex 1. A 2. B 3. C 4. 5. E 6. F 7. G 8. H TGA F AATAAA ↑ H
- 42/ The port mutaton that doesn't produce a change in the amano and sequence of proten is known as A Silent mutation B Chromosomal mutation C Frame-shift mutation D Non-sense mutation E Missense mutation5 Terminator Transcription stops 5' Transcription continues Antiterminator 4. pt) Among the structurally simplest riboswitches are the two so-called purine riboswitches, one of which responds to guanine, and the other to adenine. The accompanying diagram shows a guanine riboswitch. Base pairing between free guanine (blue) and a particular cytosine residue within the aptamer determines the riboswitch conformation. a) What condition in the cellular environment would favor each of the riboswitch configurations?that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification Post-translational modification Reactive (R) group Trans-acting C Operon Nuclear localizationsignal Double-stranded RIA Shine-Dalgarno sequence Kozak sequence E site on ribosome Ps p riboeE8R3) A site on ribosome Constitutive F Allosteric transition Inducer ll ol g Repressor T G H. Nonsense codon Frameshift mutation W Chaperones Found upstream of AUG codons in bacteria, this binds the 3' end of the 16S rRNA of the 30S ribosomal subunit Addition of chemical groups to amino acids in a polypeptide Hidigel elnw eesel Poly (A) polymerase enzyme uses ATP as substrate to add a string of A nucleotides on to the 3'-OH of MRNA Jeiheg 1ot eninoaso uoy nielgxe 10 anoitamusas Tuoy elsta otehgo1qqs eedW 4 Is produced when both strands of DNA encoding a gene are transcribed simultaneously A class of proteins that arrest incorrectly or incompletely folded proteins 6. Occurs when allolactose binds Lacl protein causing…