One technique for staining bacteria for viewing under the microscope is called the Gram stain. In this technique, alcohol is used a it degrades the outer membrane found in some bacteria. What chemical component of the cell does alcohol affect?
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- which statemts pertin to Gram stain, acid-fast stain, or spore stain. please group statements with the correct term they pertain to Here are the descriptions: -does not require heat to be used in staining process -distinguishes between active metabolic cells and and dormant structures -a chemical is used as a mordant -differentiates cell with high lipid content in cell wall -uses carbol fuchsin, acid alcohol and methylene blue uses malachite green -differentitates cells based on thickness of peptidoglycan layer -result is purple and red/pink cells -important diagnostic tool in mycobacterium infectionsCellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a bacterial preparation. Determine metabolic properties of a cell. To determine the shape of the bacteria in the culture. To determine what type of cell wall the bacteria has (e.g. gram positive or Gram negative) Identify specific features of a cell (spores, flagella etc)In an acid-fast staln, acid fast bacteria retain their primary stain after the decolorization step with acid alcohol because of the In their cell walls. O peptidoglycan O lipopolysaccharldes teichoic acids O phospholipids O mycolic acid
- In the Gram stain, gram-negative bacteria appear red for all the following reasons EXCEPT: They stain with safranin because they are colorless at the step where it is added Safranin is a red stain None of the other four answers (all are true) The peptidoglycan layer in their cell walls is too thin to retain the crystal violet-iodine complex when the decolorizer (alcohol) is added The gram-negative cells' outer lipopolysaccharide layer (outer membrane) prevents their being stained by crystal violet in the first stepArchaea with cell walls consisting of a thick, homogeneous layer of complex polysaccharides often retain crystal violet dye when stained using the Gram-staining procedure. Suggest a reason for this staining reaction.Suppose you are staining three identical smears of the same bacterial culture with three different basic simple stains: carbol fuschin, crystal violet and methylene blue. You observe each stained preparation under oil immersion at 100X. which of the following bacterial characteristics would you expect to be different between each of the stains as you view them under the microscope. Bacterial arrangement Bacterial shape Size of cells The color of the bacteria
- Several students argued about why Gram-positive cells stain purple with the Gram stain whereas Gram-negative cells stain pink. Which explanation is correct? Multiple Choice Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-negative cells prevents the crystal violet from being washed out Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Safranin stains lipopolysaccharide, and because only Gram-negative cells have lipopolysaccharide, those cells look pink. Alcohol dehydrates peptidoglycan; the thick layer of dehydrated peptidoglycan in Gram-positive cells prevents the crystal violet from being washed out. Crystal violet specifically stains peptidoglycan, and because Gram-positive cells have a thicker peptidoglycan layer those cells look purple. Alcohol destroys lipopolysaccharide in Gram-negative cell walls, allowing safranin to enter the cells and staining them pink.An acid-fast stain can be used to detect protective coverings found in using light microscopy. organisms incorporating mycolic acid in the cell wall organisms forming endospores organisms with capsules organisms with Gram-negative cell wallsThe picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no ( negative and positive) And explain? (Is there growth or not?) Agar
- why does are commonly used to stain cytoplasm granules blueWhat color would the bacterial cells have when viewed microscopically after the following treatments: Gram Reaction Ammonium Oxalate Gram lodine Ethyl Alcohol Safranin Crystal Violet Gram-positive Gram-negativeMembrane filters are probably used for sterilization because filters with smallest pore size do not clog easily. many materials are destroyed by heat sterilization they are used to sterilize only very small amounts of liquid they denature proteins