One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 5C ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is ug/ml, considering the dilution factor of - (Note: ug= microgram, ul- microliter). O 70/10 O 140/20 O 280/50 O 560/100

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
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One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 50
ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The
diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is
ug/ml, considering the dilution factor of
(Note: ug microgram, ul- microliter).
70/10
140/20
280/50
O 560/100
Transcribed Image Text:One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 50 ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is ug/ml, considering the dilution factor of (Note: ug microgram, ul- microliter). 70/10 140/20 280/50 O 560/100
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