Olivia developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown below. 2 3 4 5 6 0 cm 1 A B C D E F G a. Determine the retention factor (Rf) values of each spot on dye C. Report your answer in 2 decimal places. b. What other dye/s are present in dye C? c. Which dye contains the least number of pigments?

Olivia developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown below. 2 3 4 5 6 0 cm 1 A B C D E F G a. Determine the retention factor (Rf) values of each spot on dye C. Report your answer in 2 decimal places. b. What other dye/s are present in dye C? c. Which dye contains the least number of pigments?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.23QAP

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A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure. Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places) What other dye/s are present in dye C and what is the dye with least number of pigments?
A new dye (DYE C) was developed and was analyzed against other dyes using paper chromatography. A solution 1:1 ethyl acetate: hexane was used as the eluent. The resulting chromatogram is shown at the photo. Determine the retention factor (Rf) values of each spot on dye C and what are the other dye/s present in dye C.
A student researcher performed a chromatographic separation of caffeine and aspartame. The retention time for caffeine, te, was found to be 200.8 s with a baseline peak width, we, of 16.1 s. The retention time for aspartame, ta, was 258.7 s with a baseline peak width, wa, of 20.6 s. The retention time for the unretained solvent methanol was 44.2 s. Calculate the average plate height, H, in micrometers for this separation, given that it was performed on a 22.1 cm long column. H = Calculate the resolution, R, for this separation using the widths of the peaks. R = 88.2 R₂5N = 3.16 Calculate the resolution if the number of theoretical plates were to increase by a factor of 2.5. 3.533 Incorrect μm
23. An aqueous mixture of two substances are analysed by both paper chromatography and high performance liquid chromatography (HPLC). In both types of chromatographies, component P of the mixture was bonded more strongly to the stationary phase than component Q. In terms of Rr and Rt, component P has the Retardation factor, Rf Retention time, R¢ А lower lower higher higher lower В lower higher higher C D 24. What is the number of structural isomers that are available for aldehyde with the chemical formula of C4H8O? А 1 В 2 С 3 D 4 25. The following reaction is initiated with UV light: alkane + chlorine → chloroalkane + hydrogen chloride What happens to chlorine in this reaction? A homolytic fission to give an electrophile B homolytic fission to give a free radical C heterolytic fission to give an electrophile D heterolytic fission to give a free radical
4. The paper chromatogram of an unknown mixture is shown below: final solvent level 5.3 ст 6.0 cm B 2.0 cm A 1.0 cm origin A. Using the measured distances find the retention factor of components A, B, and C. B. If the paper was run using hexane as the solvent, (a) which compound is the most polar? (b) the least polar? Explain your answer. C. What would you expect to happen to the Rf values if acetone was used instead of hexane as the solvent?