Mutated DNA Template Strand #2: 3’-T A C G G A C T G A C G A T C-5’ Complementary DNA sequence: mRNA sequence transcribed from template: Amino acid sequence of peptide: Type of mutation:
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Mutated DNA Template Strand #2: 3’-T A C G G A C T G A C G A T C-5’
Complementary DNA sequence:
mRNA sequence transcribed from template:
Amino acid sequence of peptide:
Type of mutation:
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- Mutated DNA Template Strand #1: 3’-T A C T G T C T G A C G A T C-5’ Complementary DNA sequence: mRNA sequence transcribed from template:Mutated DNA Template Strand #1: 3’-T A C T G T C T G A C G A T C-5’ Amino acid sequence of peptide: Type of mutation:ENE 2: Nose Style DNA Template Strand ATG- GGG- CTT- CTC- TTT MRNA tRNA Amino Acids APPEARANCE
- SPLIT DNA MRNA TRNA Codon Anticodon Amino Acid A T C A T T A T C T A G ADNA DNA Leading strand mRNA tRNA Amino Acid A T G A A A G T C A T T T A GDNA Replication: 1. Write in the new (complimentary) strands for each of the two halves of the DNA molecule below to show what the two semi-conserved strands would look like. A G T A C C G G G C A A A C T G C A T T G T G I I I I I I I I I I I I I I I I I I I I I I I I I I T C A T G G C C C G T T T G A C G T A A C A C old strand A G T A C C G G G C A A A C T G C A T T G T G new strand: new strand: old strand T C A T G G C C C G T T T G A C G T A A C A C Gene Expression: 2. Use your DNA strand to construct a part of a messenger RNA molecule. DNA A G T A C C G G G C A A A C T G C A T T G T G mRNA:
- Protein synthesis is a complicated process involving DNA being transcribed to RNA, which is then translated into amino acids. Complete the DNA-to-amino acid table for three consecutive codons with the appropriate nucleotides and amino acids using a codon table. Nucleotide and amino acid options can be used multiple times or not at all. 5' to 3' DNA strand 3' to 5' DNA strand transcribed mRNA tRNA anticodon amino acid arginine cysteine leucine T A U A T G A U arginine leucine A proline T A U Answer Bank A G G с с T T A A U glutamic acid U G C G с с glutamic acid G G G с с G C arginine C G с GBelow is the 5’–3’ strand of a double-stranded DNA molecule with the following nucleotidesequences (all belong to an exon):5’ C C T A T G C A G T G G C C A T A T T C C A A A G C A T A G C 3’ 1. If the above DNA strand is the template (antisense) strand and the DNA molecule is transcribed that produced a functional mRNA. Assuming there are no mutations, the said mRNA is then brought to the site of protein synthesis,a. what would be the amino acid sequence of the synthesized polypeptide chain?b. how many possible kinds of tRNA molecule that will bring the 2nd amino acid observing wobble hypothesis? List down their anticodons.ANALYSIS ANALYSIS: A DNA strand undergoes all the process included in the central dogma. The DNA strand used as a template is given below: Parent strand DNA: DNA daughter strand: 5-AGA-ACT-AAA-CТА-ТСG-СTT-CGT-3' hnRNA: MRNA: original protein: mutated mRNA: mutated protein: second letter A G UUUPhe UCU) UCC UAU UGU Cys UUC U UUA UAC Tyr UGC Ser UAA stop UGA stop| A UAG stop UGG Trp UCA Leu UUG UCG G CUU CCU CAU CGU His CAC САА CUC CC CGC Leu Pro Arg A CUA ССА CG CGA Gln CAG SO AAU CUG CGG AUU ACU AGU AGC Ser A Asn AUC lle A AUA ACC AAC Thr AAA AAG Lys GAU АСА AUG Met ACG AGG Arg GUU GCU GGU U Asp GUC GGC GCC GCA GCG GAC Val GUA Ala Gly A GAG Glu GGG GAA GGA GUG G Translating the unmutated MRNA that was obtained after splicing, the original protein sequence will be? The original protein is [A] (For your answer, use the one-letter symbol (all caps) for the amino acid residues separated by dashes, e.g. C-A-S-H). first letter third letter
- AKS 5b: Which statement is correct regarding the semiconservative nature of DNA? * The semiconservative nature of DNA allows for genetic stability in somatic gene production MRNA operates as a template to allow DNA to replicate itself using ribosomes The structure of the phosphate group on the DNA molecule direct the correct nucleotides into place during replication Nucleotides in each original strand serve as a template for the new strand to be made AKS 5b: Which model accurately represents the semi-conservative nature of DNA replication? * * AA AA AA AA АВ ВА AA BB AA AA АВ АС Figure A Figure B Figure C Figure DBase on the coding strand of DNA: ATG GGA ATT CGC What is the sequence of the complementary template strand of DNA that pairs with the coding strand?DNA strand below: 3’ T A C A T G C C G A A T G C C 5’ Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA. Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain. 1. Give the discussion of the entire procedure