Minimize pipette handling Questions : How important is it to transfer the proper liquids in their proper containers? What will happen if you placed the wrong volume of liquids
Minimize pipette handling Questions : How important is it to transfer the proper liquids in their proper containers? What will happen if you placed the wrong volume of liquids
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
Problem 6Q
Related questions
Question
ANSWER THE QUESTION PROPERLY
INTRODUCTION :
Pipetting is one of the most important skill of a cell and molecular biologist. This technique is needed because you will be working in the molecular scale. You will be measuring minute amount of liquid and other toxic / and non-toxic materials. You will also be working with DNA samples and other materials that must be transferred from one micro-container into another. This activity will help you develop your skills in pipetting as a major skill to be acquired in this subject matter.
As your laboratory’s demand for accuracy and precision grows, so does the importance of understanding and developing an optimal pipetting technique. Walk through the following 12 steps that will bring your pipetting to the next level, and see how many you have already mastered and integrated as part of your everyday pipetting routine.
Step 1: Pre-wet the pipette
Step 2: Immerse the tip to the proper depth during aspiration
Step 3: Pause consistently after aspiration
Step 4: Use consister plunger pressure and speed
Step 5: Pull the Pipette straight out
Step 6: Examine the tip BEFORE dispensing a sample
Step 7: Examine thr tip AFTER dispening a sample
Step 8: Use standard mode pipetting
Step 9: Use the appropriate pipette
Step 10: Use thr correct pipette tip
Step 11: Work at ambient temperature equilibrium
Step 12: Minimize pipette handling
Questions :
How important is it to transfer the proper liquids in their proper containers? What will happen if you placed the wrong volume of liquids?
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