m doing lab report for thin layer chromatography right now, but I'm struggling with a question about how the polarity affect separation. Please help me answer and explain it. Thank you so much
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I'm doing lab report for thin layer chromatography right now, but I'm struggling with a question about how the polarity affect separation. Please help me answer and explain it. Thank you so much!
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- Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.
- Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC. -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.How else can chromatography be used? None of these answers are correct. To wash (or remove) unwanted solvents from a mixture. To identify or exclude components in a mixture. To generate IUPAC names for hydrocarbons.Can you use chromatography to purify a mixture? Why?
- The uses of thin layer chromatography are: 1) To identify unknown compounds 2) Determine purity of products 3) Analyze reaction mixtures 4) To waste time in lab 5) Determine the melting point of the product U 6) Put an astronaut on the moon.An example of planar chromatography is: Column chromatography HPLC GLC TLCConsider four distinct physical properties: boiling point, melting point, solubility, and density. Which of these physical properties do you think allows thin layer chromatography (TLC) to work? Choose one then, explain your reasoning.