Lid (Little imaginal discs) is a gene located on chromosome 2L and FRT40A refers to an FRT sequence inserted at the 40A (cytological location). Tubulin (Tub) refers to a promoter that can drive target gene expression in all the cell types. Gal80 is an inhibitor of Gal4 that blocks Gal4’s ability to activate transcription. Determine whether the following statements are true or false for flies with the following genotype: y w HsFLP; lid, FRT40A / P{Tub-Gal80}, FRT40A; P{Tub-Gal4} P{UAS-GFP} / + A. The fly will express Gal4, which will activate GFP in all the somatic cells without heat shock. B. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at FRT40A. However, lid mutant clone will be not induced because it is located closer to the centromere than FRT40A. C. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at FRT40A, resulting in the generation of homozygous lid mutant clones and twin spots that are homozygous for P{Tub-Gal80} insertion. D. After heat shock, the lid mutant clones generated can be identified by the absence of GFP expression. E. After heat shock, the lid mutant clones generated can be identified as the GFP expressing cells.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Lid (Little imaginal discs) is a gene located on chromosome 2L and FRT40A refers to an FRT sequence
inserted at the 40A (cytological location). Tubulin (Tub) refers to a promoter that can drive target gene
expression in all the cell types. Gal80 is an inhibitor of Gal4 that blocks Gal4’s ability to activate transcription.
Determine whether the following statements are true or false for flies with the following genotype:
y w HsFLP; lid, FRT40A / P{Tub-Gal80}, FRT40A; P{Tub-Gal4} P{UAS-GFP} / +
A. The fly will express Gal4, which will activate GFP in all the somatic cells without heat shock.
B. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at FRT40A.
However, lid mutant clone will be not induced because it is located closer to the centromere than
FRT40A.
C. After heat shock, FLP recombinase will be induced, which will induce mitotic recombination at FRT40A,
resulting in the generation of homozygous lid mutant clones and twin spots that are homozygous for
P{Tub-Gal80} insertion.
D. After heat shock, the lid mutant clones generated can be identified by the absence of GFP expression.
E. After heat shock, the lid mutant clones generated can be identified as the GFP expressing cells.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
