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A:
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Q: True/ False
A: TRUE
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Q: Use the karyotype to answer the following questions
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- The decolorizing agent damages the peptidoglycan layer. Why is this important to consider when deciding how long to decolorize a specimen as part of the
Gram stain procedure?
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- In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic?What is the purpose of each reagent in gram-staining? Primary stain Mordant Decolorizing agent Counterstain
- The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. SafranineExplain what happens to Gram-positive and Gram-negative bacterial cells at each step of the Gram-staining procedure.Why should a young culture be used in gram staining rather than an old culture?b. Why is it necessary to decolorize only for a few seconds
- The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixedif an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?
- For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?Which step of the gram stain procedure is most critical?why?The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stain