L of Microscopes: 1. If you have a standard ocular lens of 10x what is the true magnification that you are looking at if you are using the 100x objective lens. What is the rule for focusing if you switch to the 40x lens? 2. Calculate the sizes of the following cells. Not all cells are viewed at 40x so use the following table to calculate the size of your cells. 4x 1 reticular unit = .025 mm 10x 1 reticular unit = .01 mm 40x 1 reticular unit = .0025 mm
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- PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.which objective lens is in place if the object you are viewing is magnified 1000x? (assume ocular lens magnification = 10x)
- What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.1. In the following table, fill in the missing information in order to calculate the Total Magnifica- tion for each objective lens. Use the information provided in the laboratory exercise to help you. Magnification of Magnification of Objective Lens Total Magnification Objective Lens Ocular Lens Lowest Power 4X Higher Power 10X Greater Power 40X Highest Power 100X 2. What would the total magnification be if a microscope had 15X ocular lenses and 4X, 10x, 45X and 95X objective lenses? Insert the correct information in the following table. Magnification of Objective Lens Magnification of Objective Lens Total Magnification Ocular Lens 4X Objective lens 10X Objective lens 45X Objective lens 95X Objective Lensgetty Ed Res • You view this cell with the 40x objective lens and it is 7 optical units wide. Use the table below to determine the size of this cell. 1 ocular unit = Objective lens 25 um 4 x 10 μη 10 x 2.5 um 40 x 100 x 1 µm
- Calculate for the calibration factor. (3 points) (Note: stage micrometer is 1 mm long with 100 divisions) EYEPIECE RETICLE 10 20 30 40 50 60 70 0 80 100 M⁹⁰ př 10 40 50 20 OPE www.fanfi |||||||| STAGE MICROMETER 60 |||||||3. Using the information provided, calculate the size of the objects viewed. Hint you will need to calculate the FOV. These cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mmThese cells are being viewed under high power. Use the length of just one cell to estimate the number of cells that can fit into the FOV. Scanning power objective = 5X; Low power objective = 40X; High power objective = 100X; Eyepiece = 10X; Low power field of view (FOV) = 1.5 mm
- 2. Calculate the field of views using the given information: A. Low power objective = 4x; high power objective= 40x; eyepiece= 10x; low power field of view (FOV)= 4.4 mm; what is the high power field of view? B. Scanning power objective= 5x; low power objective=10x; eyepiece= 5x; scanning power field of view (FOV)= 3.5 mm; what is the low power field of view?5)The simulation asks you about the TOTAL magnification of when you use the 40x objective. This total magnification is based on the the amount of magnification provided by the ocular lens in the eyepiece, as well as the magnification of the objective. Based on the answer given in the simulation, what is the magnification of the ocular lens? (enter the number only) Answer valueDiseussion: What is the microexamination, why it is used and what is the difference between macro and microexamination? 2. state the purpose of the followings: Coarse adjustment knob, b. Fine adjustment knob, e. Iris diaphragm. d. Objective lens. e. Ocuiar or eye lens. Lengith, e 3. If the approximate magnification when using an objective lens with a focal Y re length of (2mm) is (800X), what is the magnification of the eye lens. 4. Calculate the focal length of the objective lens for a microscope with an approximate magnification of (1000x) and an eye lens magnification of (10x). * 5. Explain briefly the microscope work mechanism to examine a specimen prépared for microexamination.