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Is a clean counter bacteria free?
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- Which of the following bacteria can survive in in temperatures ranging from 390F (40C)---to 990F (37OC)?What is a chemostat? a) a chemical used as a substrate for growth of microbial cells b) a batch culture that uses a defined medium for growth c) a system used for the continuous culture of microbial cells d) a nutrient that is able to control the growth rate of a bacterial cultureWhat general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria?
- Explain why it is essential that bacteria cultures be sterilized in an autoclave.Why do microorganisms grow more slowly as the pH deviates from their optimum growth?Define growth. Why does the lag phase in some bacteria is short or absent and the log phase of the bacterial growth is preferred in any industrial process?
- Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.). Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n): A) Selective medium. B) Differential medium. C) Enrichment culture. D) Selective and differential medium. E) Differential and enrichment culture.How does E coli grow in liquid medium?
- 6) The autoclave desired Temperature to kill bacterial spores must be left for at least ____ in order to effectively kill the bacterial spores: a) 5 mins b) 10 mins c) 15 mins d) ½ hr e) 1 hr.7) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 PSI b) 15 Joules c) 15 KSI d) 15 Lbs e) 15 Atmospheres8) The antibiotic that inhibits the cell wall formation through the -Lactam ring is/are:a) Vancomycin b) Tetracycline c) Penicillin d) Cephalosporin e) c & d9) The sum of all chemical reactions in your body defines: a) anabolism b) metabolism c) catabolism d) Cellular Respiration e) Biosynthesis10) If in a bacterial growth medium waste product accumulates than the bacterial growth stage will be called: a) Death b) Convalescence c) Lag phase d) Log e) StationaryWhat steps are necessary to ensure the sterility of materialcontaminated with bacterial endospores?The correct order of steps when purifying a protein are: Group of answer choices 1)Growing bacterial culture for fusion protein; 2)Harvesting IPTG-induced cultures; 3)Lysing bacterial cell; 4)Removing insoluble debris; 5)Using Affinity Chromatography to purify protein. 1)Growing bacterial culture for fusion protein; 2) Breaking open bacterial cells; 3) Removing insoluble debris: 4) Harvesting IPTG-induced cultures; 5)Using Affinity Chromatography to purify protein 1) Growing bacterial culture for fusion protein; 2) Lysing bacterial cells; 3) Harvesting IPTG-induced cultures; 4) Removing insoluble debris: 5)Purifying protein by affinity chromatography 1)Growing bacterial culture for fusion protein; 2) Removing insoluble debris; 3) Harvesting IPTG-induced cultures; 4)Breaking open bacterial cells; 5)Purifying protein by affinity chromatography