In the next experiment, MCF-7 cells were treated or not with indibulin and the effect of this drug on the cell DNA was analyzed as described on Figure 4. Control 150 nM 300 nM 600 nM 75 60 DNA 45 30 PH3S10 15 Merged control 150 nM 300 nM 600 nM Figure 4: MCF-7 cells were incubated for 48 h with the dilution buffer of indibulin (Control) or various concentrations of indibulin, then stained with an antibody specific to phosphohistone H3 (name of the antibody: S10) (green channel), a mitotic marker, and with Hoechst 33258 (blue channel). Scale bar = 10 um. The histogram shows the percentage of phosphohistone H3 (S10)- positive cells, when cells were treated with the indibulin dilution buffer or various concentrations of indibulin. Three hundred cells were counted in each treatment condition, and the graph represents the average data from three independent experiments. Error bars represent the standard deviations. Note: the Hoechst 33258 reagent is an intercalating molecule that can pass membranes, whatever the state of the cell (i.e. alive or dead). % of Phosphohistone H3 s10 positive cells

In the next experiment, MCF-7 cells were treated or not with indibulin and the effect of this drug on the cell DNA was analyzed as described on Figure 4. Control 150 nM 300 nM 600 nM 75 60 DNA 45 30 PH3S10 15 Merged control 150 nM 300 nM 600 nM Figure 4: MCF-7 cells were incubated for 48 h with the dilution buffer of indibulin (Control) or various concentrations of indibulin, then stained with an antibody specific to phosphohistone H3 (name of the antibody: S10) (green channel), a mitotic marker, and with Hoechst 33258 (blue channel). Scale bar = 10 um. The histogram shows the percentage of phosphohistone H3 (S10)- positive cells, when cells were treated with the indibulin dilution buffer or various concentrations of indibulin. Three hundred cells were counted in each treatment condition, and the graph represents the average data from three independent experiments. Error bars represent the standard deviations. Note: the Hoechst 33258 reagent is an intercalating molecule that can pass membranes, whatever the state of the cell (i.e. alive or dead). % of Phosphohistone H3 s10 positive cells

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter8: Dna Structure And Function

Section: Chapter Questions

Problem 5DAA: HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952...

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