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In the experiment described above, specifically state what each of these parts of the experiment was:
- Independent variable
- Dependent variable
- Control group
- Experimental group
- One controlled variable
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- When 1 million cells of a culture of haploid yeastcarrying a met− auxotrophic mutation were plated onpetri plates lacking methionine (Met), five coloniesgrew. You would expect cells in which the originalmet− mutation was reversed (by a base change back tothe original sequence) would grow on the media lackingmethionine, but some of these apparent reversions couldbe due to a mutation in a different gene that somehowsuppresses the original met− mutations. How wouldyou be able to determine if the mutations in your fivecolonies were due either to a precise reversion of theoriginal met− mutation or to the generation of a suppressor mutation in a gene on another chromosome?You have identified five genes in S. cerevisiae that are induced when the yeast are grown in a high-salt (NaCl) medium. To study the potential roles of these genes in acclimation to the growth in high-salt conditions, you wish to examine the phenotypes of loss- and gain-of-function alleles of each. How will you do this?In this activity you will analyze the results of experiments that investigate nutritional requirement of several mutant strains of yeast. The mutations in these strains cause a nutritional requirement for an amino acid, such that the strains will not grow in media that lack one specific amino acid. Any mutant that has a nutritional requirement is called an auxotroph, and is incapable of growing in a "minimal medium" containing only a carbon source (e.g., glucose), a simple nitrogen source (e.g., ammonium sulfate), and various salts and minerals. Such strains can be supported on a medium supplemented with only the missing nutrient or on a "rich" medium that contains amino acids, vitamins, nitrogenous bases, etc. (often in the form of an extract from yeast). The wild-type individual that can synthesize the metabolic component is a prototroph, and is capable of growth on minimal medium. The mutant strains in this activity are unable to synthesize tryptophan, lysine, or histidine; one…
- An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?One hallmark of cancer cells is their ability to divideindefinitely, in contrast with most normal somaticcells that undergo senescence after 30 to 50 generations of divisions. We saw in this chapter that one reason for this difference is that many cancer cellsexpress the telomerase enzyme that can mediate telomere lengthening.Interestingly, about 15% of tumors do not expresstelomerase. Instead, they lengthen their telomeres byan alternative pathway. Tumor cells of this class appear to have telomeres that are highly heterogenous inlength; some telomeres have many more TTAGGGrepeats than others.a. Diagram an event involving homologous recombination that would allow some telomeres in thesecells to become longer. What feature(s) of telomeresmake(s) such homologous recombination possible?b. Does this recombination need to occur between homologous telomeres (that is, telomeres of the samearm of the same chromosome)? If such recombination could occur between nonhomologous telomeres, how might…The results of the fluctuation test (Fig. 7.5) were interpreted to mean that different numbers of mutantbacteria preexisted in each of the 11 culture tubes because the mutations arose spontaneously at differenttimes during the growth of each culture. However, another possibility is that the differences in the numberof colonies on the plates are simply due to differencesin the ability of the petri plates to support the growthof colonies. For example, perhaps the selective agentor the nutrients in the media were not evenly distributed in the molten agar poured into the petri dishes.What experiment could you do to determine whetheror not differences in the petri plates were a factor inthe experiment?
- You have isolated a strain of mutant yeast cells that divide normally at 25 degrees Celsius but cannot ente4 M phase at 37 degrees Celsius. You found that the gene for M cdk is not mutated. Which of the following temperatures sensitive mutations could be responsible for the.behavior of this strain of yeast? Inactivated of an enzyme that normally degrades m cyclin; Inactivated of a protein that normally triggers the synthesis of m cyclin; Inactivated of a protein kinase that normally Inactivate the cdk; none 2. Which of the following statements about the anaphase promoting complex is false? It promotes the degradation of proteins that activates mitosis; it inhibits m cdk activities; it becomes active at the very beginning of metaphase; it is activated by m cdk 3. During cancer development:.? Cells accumulate mutations in a fixed order which results in increase rate of cell division; the inheritance of mutations caused by dna damage is essential for cancer progression; there is typically…why does a clone of cells produceonly one type of G-6-PD enzyme? What would you expect to happen if a clone was derived from an early embryonic cell? Whydoes the initial sample of tissue produce both forms of G-6-PD?You have just discovered a protein in mice that may be aneffective cure for cancer, but it is present only in tiny amounts.Describe the steps you would use to produce this protein intherapeutic amounts. Which host would you want to clonethe gene into and why? Which host would you use to expressthe protein in and why?
- A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colony morphologyis strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology? Also, describe what happens when a nonsense mutation is introduced into the gene encoding transposase within a transposon and why is it more likely that insertions or deletions will be more detrimental to a cell than point mutations?The family of a sixth-grade boy in Palo Alto, California, wasinformed by school administrators that he would have to transferout of his middle school because they believed his mutation ofthe CFTR gene, which does not produce any symptoms associatedwith cystic fibrosis, posed a risk to other students at the schoolwho have cystic fibrosis. After missing 11 days of school, a settlementwas reached to have the boy return to school. What ethicalproblems might you associate with this example?Some people have a genetic predisposition for developing priondiseases. Examples are described in Table 25.6. In the case ofGerstmann-Straüssler-Scheinker disease, the age of onset istypically 30–50 years, and the duration of the disease (whichleads to death) is about 5 years. Suggest a possible explanationwhy someone can live for a relatively long time withoutsymptoms and then succumb to the disease in a relativelyshort time.