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If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what that indicate about the bacterial culture?
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- If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what might that indicate about the bacterial culture?What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?If the seven-digit code that is tabulated cannot be found in the Profile Determination Table, what might that indicate about the bacterial culture?
- If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar plate gave rise to 229 distinct colonies. What was the concentration of bacteria in the original, undiluted suspension?Why do the results for the confirmed test represent faecal coliform in the analysis of water using the MPN procedure?
- On the basis of the appearance of this place, what are you testing for? When you added a reagent to this plate, these bubbles appeared. What is the name of this reagent and what are those bubbles?a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteriaHow will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?
- A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plateYou have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different?b) What is “coating buffer” for an ELISA? Does the buffer molecule usedmake sense based on the desired pH?In an ELISA procedure the samples are incubated and the ELISA plate iscovered with parafilm and placed in a humidified chamber to preventevaporation of the small liquid volumes in the wells.c) How would your results change if you did not incubate in a humidifiedchamber?