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Identify some advantages and limitations of using HPLC, and explain
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- Which is better HPLC system Agilent 1290 Infinity II LC or Nexera Series UHPLC? ExplainList the desirable characteristics of HPLC detectors.Following are infrared spectra of 2-methyl-1-butanol and tert-butyl methyl ether. Assign each compound its correct spectrum. Micrometers 2.5 100 3. 4. 9. 13 8 10 11 12 14 15 20 90 80 70 50 40 30 20 10 4000 3600 3200 2800 2400 2000 1800 1600 1400 1200 1000 800 600 400 Wavenumber (cm-1) Transmittance (%)
- Why is GSC not used nearly as extensively as GLC?Lrdocaine USP (ATR) 95- 90 85 80 75 70 65 60 55 50 45 Lidocaine 40 4000 3500 3000 2500 2000 1500 1000 Wavenumbers (cm-1) %Transmittance IZ 3243.36 3021.30 2968.47 2921.13 2799.39 1661.66 1593.98 1491.43, 1449.061 422.12,384.681371.62 1291.03264.55 1206.99 1165.931119.56 1091.78072.77. 1034.33 970.79986.39 917.91, 885.23 763.76 703.94 615.01599.90please quickly ,within 1hour thanks ! If it takes longer than an hour, i don't need the answer (1) List the detectors ofHPLC. Describe the advantage and disadvantage for two wideused detectors
- Binary mixtures of NiO and Mo were prepared containing 20,50,and 80 weight percent of NiO. 20g of Al (internal standard) were added to 70 g of these mixtures and to 70 g of an unknown mixture of NiO and Mo. The intensity of suitable reflections were recorded. NiO(wt%) Mo(wt%) I(NiO) I (Mo) I (Al) I(NiO)/I(Al) I(Mo/IAl) 20 80 73 639 246 50 50 196 423 261 80 20 508 271 423 Unknown 227 364 263 Calculate I(NiO)/I(Al) and I(Mo)/I(Al) and plot vs % NiO and % Mo respectively Determine composition of unknown : wt % NiO and wt % Mo in unknown mixture. What is the molar absorptivity when the percent transmittance is 56.2%, the pathlength is 2.00 cm, and the concentration is 0.0000748 M?Why are some of the detectors listed unsuitable for HPLC?