I have: DMEM:F12 800X ITS 1.07 g/ml Linoleic acid Nu-serum (pure solution) BSA (pure powder) How much of each would I need to make 500ml of (1x ITS, 7.49 ug/ml linoleic acid, 4% v/v Nu-serum, 0.15% W/V BSA, and DMEM:F12 as the solvent)?
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I have:
DMEM:F12
800X ITS
1.07 g/ml Linoleic acid
Nu-serum (pure solution)
BSA (pure powder)
How much of each would I need to make 500ml of (1x ITS, 7.49 ug/ml linoleic acid, 4% v/v Nu-serum, 0.15% W/V BSA, and DMEM:F12 as the solvent)?
Step by step
Solved in 3 steps
- If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g BSA, and DMEM:F12 as my solvent, how best would I make this solution and what tools would be the most accurate to use?You have prepared the following serial dilution using tryptone broth:d1: 0.1ml culture + 9.9 ml brothd2: 300µl d1 + 4.7 ml brothd3: 0.2ml d2 + 9800 µl broth1) Write your dilutions as a rational number AND in scientific format? What was your total dilution (in scientific notation)?2) What was the original concentration of your culture if you spread 0.1 ml of d3 and counted360 cfu?The doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show work
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- A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?
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