I) Choose the Correct Answer from the Multiple-Choice List (10) 1. Which of the following is not a common type of packing for reversed-phase HPLC? C. Diol D. phenyl A. C18 B. C8 2. Gradient elution is a separation that employs : a. a single solvent of constant composition. b. solvent mixture of constant composition. c. two or more solvent systems that differ significantly in polarity. d. both (a) and (b). 3. The most useful bonded-phase coatings in HPLC are . : a) glass b) alumina c) inert silica d) siloxanes 4.Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b) Hexane c) Acetonitrile d) Methanol e) water 5. It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV detector c. fluorescence detector d. UV-visible detector 6. High performance liquid chromatography (HPLC) can be used to : a. identify the various pigments from a leaf extract. b. separate types of organic pesticides. c. determine the mercury content of a fish sample d. all of the above are correct 7. The compound eluted last and retained more in Normal phase Chromatography are: A. Polar compounds B. Non- polar and Polar compounds C. Non polar compounds D. ionic compounds 8. Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the above 9. Which of the following solvents is used in normal phase elution in HPLC? a) Ethanol: Water b) Hexane c) Acetonitrile CH;CN d) Methanol 10. which of the followings is the universal detector used in HPLC: a) Fluorescence b) IR c) FID d) ECD e) RI 11. Analyte that are extremely Hydrophobic (or) incompatible with water, a mobile phase that contains no water is used. The separation is termed as : a. Reversed phase chromatography b. Normal phase chromatography c. Non- aqueous Reversed phase chromatography d. Aqueous Normal phase chromatography 12. This detector is not used in HPLC: a) ECD b) MS c) Fluorescence d) RI
I) Choose the Correct Answer from the Multiple-Choice List (10) 1. Which of the following is not a common type of packing for reversed-phase HPLC? C. Diol D. phenyl A. C18 B. C8 2. Gradient elution is a separation that employs : a. a single solvent of constant composition. b. solvent mixture of constant composition. c. two or more solvent systems that differ significantly in polarity. d. both (a) and (b). 3. The most useful bonded-phase coatings in HPLC are . : a) glass b) alumina c) inert silica d) siloxanes 4.Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b) Hexane c) Acetonitrile d) Methanol e) water 5. It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV detector c. fluorescence detector d. UV-visible detector 6. High performance liquid chromatography (HPLC) can be used to : a. identify the various pigments from a leaf extract. b. separate types of organic pesticides. c. determine the mercury content of a fish sample d. all of the above are correct 7. The compound eluted last and retained more in Normal phase Chromatography are: A. Polar compounds B. Non- polar and Polar compounds C. Non polar compounds D. ionic compounds 8. Derivatiztion of sample is carried out to: a) Improve analyte peak shape analyte resolution b) Increase the detector response c) Improve d) All the above 9. Which of the following solvents is used in normal phase elution in HPLC? a) Ethanol: Water b) Hexane c) Acetonitrile CH;CN d) Methanol 10. which of the followings is the universal detector used in HPLC: a) Fluorescence b) IR c) FID d) ECD e) RI 11. Analyte that are extremely Hydrophobic (or) incompatible with water, a mobile phase that contains no water is used. The separation is termed as : a. Reversed phase chromatography b. Normal phase chromatography c. Non- aqueous Reversed phase chromatography d. Aqueous Normal phase chromatography 12. This detector is not used in HPLC: a) ECD b) MS c) Fluorescence d) RI
Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 33.2QAP
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