Here you see the gel electrophoresis of PCR reactions of the pMCT118 VNTR locus of 4 individuals (lanes 1-4). The outermost lanes show a DNA size standard (100bp ladder), with the fastest migrating band equal to 100bp and each successive band 100bp larger (200, 300, 400, 500, 600, etc.) Which is the smallest band of the DNA size standard? Approximately what size are all of the bands seen in lanes 1-4? Which of these four persons is homozygous for the VNTR locus? Which two individuals cannot be discriminated by this PCR

Aquaculture Science
3rd Edition
ISBN:9781133558347
Author:Parker
Publisher:Parker
Chapter4: Management Practices For Finfish
Section: Chapter Questions
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Here you see the gel electrophoresis of PCR reactions of the pMCT118 VNTR locus of 4 individuals (lanes 1-4). The outermost lanes show a DNA size standard (100bp ladder), with the fastest migrating band equal to 100bp and each successive band 100bp larger (200, 300, 400, 500, 600, etc.)

  1. Which is the smallest band of the DNA size standard?
  2. Approximately what size are all of the bands seen in lanes 1-4?
  3. Which of these four persons is homozygous for the VNTR locus?
  4. Which two individuals cannot be discriminated by this PCR?
Тop
12 3 4
В
A
Bottom
1. 4
Transcribed Image Text:Тop 12 3 4 В A Bottom 1. 4
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