Give specific procedure ( tell the measurement needed ) based on this using yeast , sugar and all home equipments only (container, oven ) Aseptic technique is a set of routine measures that are taken to preventcultures from being contaminated by unwanted microorganisms. Examplesof aseptic techniques are:-Sterilized all containers used for inoculation to avoid any possiblei.contamination.-Cleaning and disinfecting work surfaces prior to use-Limiting the duration that cultures or media are uncapped and exposed tothe air-Keeping containers closed whenever possible-Effectively sterilizing inoculating loops/tools that comes into contact withcultures or media, and-Avoiding breathing on cultures or sterile instruments/tools.Preparation of medium with carbon sourcePreparation of additional nutrientPreparation of inoculum (can be either yeast or natural bacterial from naturalsources for instance dry yeast or fruit bacteria or any other kind)Inoculation techniqueSampling growth at initial time: t = 0 hourSampling growth at you selected interval of time for instance every 5 hoursor etc. Please choose accordingly so as to ensure at least 5 minimum datais collected considering lag phase, growth phase, stationary phase, anddeath phase.i.iii.iv.V.vi.vii.viii.Qualitative observationQuantitative observation for this case cell dry weight (CDW)ix

This procedure outlines a simple method to study yeast growth and measure cell dry weight using…

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Give specific procedure ( tell the measurement needed ) based on this using yeast , sugar and all home equipments only (container, oven )

Basic Clinical Lab Competencies for Respiratory Care: An Integrated Approach

5th Edition

ISBN:9781285244662

Author:White

Publisher:White

Chapter19: Equipment Processing And Surveillance

Section: Chapter Questions

Problem 5SEPT

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Question

Give specific procedure ( tell the measurement needed ) based on this using yeast , sugar and all home equipments only (container, oven )

Aseptic technique is a set of routine measures that are taken to prevent
cultures from being contaminated by unwanted microorganisms. Examples
of aseptic techniques are:
-Sterilized all containers used for inoculation to avoid any possible
i.
contamination.
-Cleaning and disinfecting work surfaces prior to use
-Limiting the duration that cultures or media are uncapped and exposed to
the air
-Keeping containers closed whenever possible
-Effectively sterilizing inoculating loops/tools that comes into contact with
cultures or media, and
-Avoiding breathing on cultures or sterile instruments/tools.
Preparation of medium with carbon source
Preparation of additional nutrient
Preparation of inoculum (can be either yeast or natural bacterial from natural
sources for instance dry yeast or fruit bacteria or any other kind)
Inoculation technique
Sampling growth at initial time: t = 0 hour
Sampling growth at you selected interval of time for instance every 5 hours
or etc. Please choose accordingly so as to ensure at least 5 minimum data
is collected considering lag phase, growth phase, stationary phase, and
death phase.
i.
iii.
iv.
V.
vi.
vii.
viii.
Qualitative observation
Quantitative observation for this case cell dry weight (CDW)
ix

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