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- From a 50% solution of dextrose, make 500 ml of a 5% solutionYou receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *How many mL of 13x stock solution would you need to make 387 mL of 1x PBS. Report your answer to 1 decimal place.
- Prepare 200 ml of a 0.125 M stock of NaCl from a 1 M concentrated stock solution of NaCl.You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquidIf you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.
- You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%How many mL of 10 x stock solution would you need to make 118 mL of 1x PBS? Report your answer to 1 decimal place.How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?
- given a stock solution of 10% dextrose, how would you make 5 mL of a 0.9% dextrose solution? find mL of stock and mL of h20You are preparing 1 Lt of TAE (Tris Acetate EDTA) gel running buffer from a 50x stock solution. How much 50 x TAE and how much water will you combine?Our laboratory has a stock of 50 X TAE (Tris-Acetate) and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).