For scenario 2, below write the following 1.the process that the sets of measurements represent. 2.suitable equations of the process. 3.the calculated rates and 4.the resulting units for the process as expressed by your calculation.
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For scenario 2, below write the following
1.the process that the sets of measurements represent.
2.suitable equations of the process.
3.the calculated rates and
4.the resulting units for the process as expressed by your calculation.
Scenario 2
Time,0 minutes:7.13mg/L O2
Time,20 minutes:13.39mg/L O2
Algal biomass:2.3g fresh mass
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- A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*KA 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K7. Make a data table for the experiments/trials. It should include reaction conditions volumes of reagents used, O2 volume produced (with correct sig figs), and observation Tor all trials. If you have made a figure displaying your results, please attach that as well | temperature Vol of Hio Vol of polato un time Oz generated 1. o'c 5ml 2ml 2. 2. 1 mL 2. 8.5 mb 37°C 4 1,8 mb 8. Analyzing your results: a. What effect does your variable have on the activity of the potato enżyme? Use numerical data to describe the how the activity changes. b. Why do you think you saw this effect? c. What specifically happened to the enzyme to cause this effect? What conclusions can you draw from the change or lack of change to the activity of the potato enzyme? d. Summarize your conclusions including numerical data on the board for your system.
- The hydrolysis of a substrate, S, by an enzyme has been studied in the lab. The following initial rates, vo, were recorded at different concentrations. [S] (M) Vo (M/min) 2.10-10-4 1.20.10-6 4.20-10-4 3.10.10-6 9.30-10-4 6.30-10-6 1.42-10-3 9.10-10-6 A. Determine the rate constants for degradation of the substrate B. What is the rate of reaction at [S] = 1.1.104 M? C. Explain, why enzymes can make reactions go faster? Does enzymes also catalyse the reverse reaction from product to substrate?After administration of 150 mg drug A in an oral tablet dosageform, the AUC was calculated to be 143.3 mg•hr/L. Afteradministration of 85 mg drug A in an IV injection dosage form,the calculated AUC was 208.6 mg•hr/L. What is the absolutebioavailability of drug A in the oral tablet dosage form? A. 39%B. 56%C. 69%D. 256%The phenol concentration of wastewater was determined from three (3) parallel determinations, giving a mean of 0.513 μg L-1 and a standard deviation of 0.05 μg L-1. Decide at a risk level of 5% (or a confidence level of 95%) whether a reference value of 0.520 μg L-1 is statistically different.
- You are given as following : 20 µl pure LDH on ice, 2.0ml of 6mM NAD+, 2.0ml of 150mM lactate, and 0.14M CAPS buffer. LDH reaction cocktail has final concentration of 1mM NAD+ and 25mM lactate in 0.14M CAPS buffer. LDH activityis measured by mixing 10 µl of LDH sample and 990 µl LDH reaction cocktail before getting ∆A340/min reading on spectrometer. (a). Describe in detail how you would prepare for your LDH reaction cocktail including how to make dilutions.Dom Add Page 0.51, what is the concentration of the unknown (in mg/mL)? Show your work for full credit. 3. (1 point) Show the answer to the following question in correct significant figures. 10.219 +3.12 = 4. (7 pts) True or False. Circle the correct answer. True or False. A blank used to zero of spectrophotometer always contains just distilled water. True or False. The mode is the most commonly occurring number in a data set. True or False. One milliliter (mL) equals 1,000,000 microliters (µL). True or False. Peroxidase activity increases when the enzyme is boiled. True or False. Enzymes are carbohydrates. True or False. Denaturing involves an enzyme breaking down into individual amino acids. True or False. Hydroxlyamine is a competitive inhibitor of peroxidase. noint) How mony uL (miorolitora) orain 222 12 Show vour work for full oredit MAR étvConsider the following experimental data from another experiment: [S] 1.5 2.00 2.50 5.00 10.00 V (No inhibitor) mmol ml¹ min¹ 0.167 0.204 0.232 0.313 0.385 V (inhibitor) mmol ml¹¹ min¹¹ 0.115 0.143 0.167 0.250 0.333 Calculate Km and V max and determine whether this inhibitor is competitive, non-competitive or uncompetitive.
- 3 A patient requires 20 mg of Oramorph. The syrup comes as 10 mg in 5 ml. How much of the syrup is required? Please give your answer as a whole number Answer: ml. Show Calculator4. Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 1/[S], (L/mol) 5What is v/Vmax ratio when [S] = 4km? %3D