Eukaryotic RNA polymerase II is used to synthesize: a. rRNA O b. tRNA O C. O d. RNA from viral DNA O e. mRNA double helical viral DNA from viral RNA
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- The RNA polymerase that requires an input of energy to initiate the process of unwinding DNA is Group of answer choices a.RNA Polymerase I and II b.RNA Polymerase I c.RNA Polymerase II d.RNA Polymerase III e.RNA Polymerase I and IIIDNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkagesRNA polymerase subunits comprising the core enzyme are a.α b.β c.δ d.σ e.ω
- Messenger RNA in eukaryotic cells is synthesized by which of the following enzymes (stronglyinhibited by even very dilute concentrations of the fungal poison a-amanitin)?A. DNA polymerase IIIB. RNA polymerase IIIC. RNA polymerase IID. DNA polymerase IE. RNA polymerase IA small section of bacterial DNA antisense strand has the following nucleotide sequence:AAT CGG TCC TCGThe tRNA anticodons for the gene sequence shown above is a. TTA GCC AGG AGC b. AAU CGG ACC ACG c. AAU CGG UCC UCG d. UUA GCC AGG AGCAssume that a mutation occurs in the gene that encodes each of the following RNA polymerases. Match the mutation with its possible effects by placing the correct letter or letters in the blanks below. There may be more than one effect for each mutated polymerase. A mutation in the gene that codes for E RNA polymerase I RNA polymerase II RNA polymerase III Possibleeffects a. tRNA is not synthesized b. Some ribosomal RNA is not synthesized c. Ribosomal RNA is not processed d. pre-mRNA is not processed e. Some mRNA molecules are not degraded f. pre-mRNA is not synthesized
- Match each enzyme name in the left column with the correct descriptive phrase in the right column. a. Topoisomerase II b. DNA ligase c. DNA polymerase y d. Reverse transcriptase i. Catalyzes most nucleotide incorporations in bacterial DNA replication ii. Cleaves RNA in a DNA-RNA hybrid molecule e. DNA polymerase I f. DNA polymerase II iii. Uses a tRNA primer in synthesis of retroviral DNA iv. Acts through an adenylylated DNA inter- mediate v. Catalyzes formation of a double-strand DNA break vi. Catalyzes mitochondrial DNA replicationMatch Column A with Column B. transports amino acids to the site of protein synthesis has anticodon loop directs amino acid sequence of proteins contains the Shine-Delgarno sequence synthesized in the eukaryote nucleus found inside the ribosome region where the RNA binds to the DNA template…In bacterial RNA polymerase, the sigma factor A. contains the catalytic activity of the polymerase B. remains part of the polymerase throughout transciption. C. recognizes promoter sites in the DNA D. recognizes transcription termination sites in the DNA E. is a subunit of DNA polymerase III
- Which of the following RNA Polymerases in Eukaryotes is responsible for producing pre-mRNA? a. RNA Pol I b. RNA Pol II c. RNA Pol III d. RNA Pol IVe. any of the above8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B. Used to test for the presence of hundreds to thousands of active genes at the same tim C. Used as a vector to replicate foreign DNA inside bacteria D. Able to cut double stranded DNA at almost any desired location for genome editing Which of the following is not an example of RNA processing. the addition of the poly-A tail to mRNA in the nucleus spliceosomes cutting mRNA in the nucleus the addition of the 3' cap to mRNA in the nucleus the joining together of exons in the nucleusPlease consider figures 1 and 2 below. 5' A B 5' -I H. D. Figure 1: Replication Figure 2: Transcription a. Which letters, A to J represent DNA strands that will be synthesized continuously? b. At which letter, A to J, are ribonucleotides being added? c. Name and describe the process that occurs in prokaryotes as the synthesized mRNA hangs free of the double helix while transcription continues. d. In what direction will the replication bubble move in figure 1 as replication continues? Rleace Ibol ancwors (a) to (d)