EcoRI 4359 Clal - BspDI 23 Hindi 29 Aatll - Zral 4284 Bki 4209 Borl 4205 EcoRV 185 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Bmtl - Nhel 229 Bami 375 Sgrl 409 Hincll 3905 Scal 3844 Bsl 3787 Bsl 3759 Banll 471 Banll 485 gl 528 Sphi 562 EcoNI 622 Sall - Accl - Hincll 651 Prul 3733 Pstl 3607- Brbi 3602 Pshl 712 Asel 3537 Eagl 909 bell 949 Nrul 972 Bsal 3433 BrDI 3420 PBR322 4,361 bp BAPI 1045 Ahdi 3361 HapMI - Bful 1063 PMI 1315 Buml 1353 PIMI 1364 Styt 1369 Aval - Bsobl 1425 PuMI 1438 Acul 3000 AlwNI 2884 Bell 2777 k 2682 Msel 1444 Bigl 1447 Pyuti 1480 rop Begl 1650 Bspl 1664 Ba 1668 Drll 2575 Peil - Al 2473 rbl 2404 Earl 2351 Xmal 2029 Prull 2064 spol - Sapl 2350 Ndel 2295 BAPI 2291 BomBI 2122 Dedl 2162 BsZ171- Aoct 2244/ Bsal 2225 TthI - PI 2217 Figure 1 If your gene of interest was inserted at the Sphi restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.
EcoRI 4359 Clal - BspDI 23 Hindi 29 Aatll - Zral 4284 Bki 4209 Borl 4205 EcoRV 185 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Bmtl - Nhel 229 Bami 375 Sgrl 409 Hincll 3905 Scal 3844 Bsl 3787 Bsl 3759 Banll 471 Banll 485 gl 528 Sphi 562 EcoNI 622 Sall - Accl - Hincll 651 Prul 3733 Pstl 3607- Brbi 3602 Pshl 712 Asel 3537 Eagl 909 bell 949 Nrul 972 Bsal 3433 BrDI 3420 PBR322 4,361 bp BAPI 1045 Ahdi 3361 HapMI - Bful 1063 PMI 1315 Buml 1353 PIMI 1364 Styt 1369 Aval - Bsobl 1425 PuMI 1438 Acul 3000 AlwNI 2884 Bell 2777 k 2682 Msel 1444 Bigl 1447 Pyuti 1480 rop Begl 1650 Bspl 1664 Ba 1668 Drll 2575 Peil - Al 2473 rbl 2404 Earl 2351 Xmal 2029 Prull 2064 spol - Sapl 2350 Ndel 2295 BAPI 2291 BomBI 2122 Dedl 2162 BsZ171- Aoct 2244/ Bsal 2225 TthI - PI 2217 Figure 1 If your gene of interest was inserted at the Sphi restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.
Chapter17: Drugs And Dissection
Section: Chapter Questions
Problem 8WS
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