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A: Thin layer chromatography is a type of chromatography.
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A: chromatography used to separate butanol from the volatile mixture
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Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?
A: In reverse phase chromatography, polar solvent is used as a mobile phase.
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A:
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A: Reversed phase chromatography have long chain alkyl chains which are non polar in nature .
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A: Given Can acetic acid be used as the mobile phase in kool aid chromatography over isopropyl alcohol
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A: as per my opinion it is option- D
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Q: 7. Which of the following compounds will elute LAST in a reverse phase chromatography? A. ONF
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Q: All chromatographic techniques can be used as separation methods. True O False
A:
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- eaninesau Question 1 Describe in detail the process and mechanism by which thin layer chromatography functions in separating the compounds in a mixture.Often, palnters mix blue and white palnt to lighten Its color. Which of the following statements below are true regarding how the presence of two pigments In a single unknown solutlon would affect the results of your experiment? O With two cations in solution, both cations can potentially react with the chosen test. O if there are two different results/ppt/color changes, there could be interference, making the identification very difficult. O For any available test that has two matching or simlar results, multiple tests will need to be performed in order to identify both pigments correctly O The two cations can react with each other to form a precipitate. Therefore, the cations are no longer available to react with the reagent added for a particular test making it very difficult, it not impossible, to identify the cations.Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?
- Explain how it is that SPE cartridges & disks allow analysts to more efficiently pre- concentrate analytes from samples where they exist at low concentrations.The following chromatogram was the output of the analysis of phytosterols in plants by gas chromatography. Beta-sitosterol Cholesterol Campesterol Stigmasterol Brassicasterol 16.5 17.0 17.5 18.0 18.5 Min Which compound had the highest affinity to the stationary phase? Lasta. Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used? HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles (1) Area (1)-14750 Arta (2)-53538 MIN HPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles (2) Mes (1)-2256 A(2)-9536 A(3)-25638 13H
- Relationship between polarity and thin layer chromatography?A Stydent attempled to puriN a moduct mxture by calumo Chromatography with a silica gel Colu mo and ethyl autate 9s The elxant. h Studint colluted lo fractunš and ran q ThC f cach fractun (Shown beland) 0000O 34 5 6 7 8 9 10 If ethanol had ban 4scd as the elyant rather than ethyl acetate, oyll yoy expect The separatiin to be better, worse, or no change?Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatography
- Which of the following statements is .14 ?incorrect Number of theoretical plates decreases .with increasing plate height Retention factor (k) directly proportional .to distribution coefficient (K) Large distribution coefficients of an analyte indicates that it will be retained .longer in the column .Selectivity factor always equal 1 The longer the time of separation, the .broader the zone (band) will beQuestion 7 Which of the following statements is true regarding liquid liquid extraction for sample's preparation. O In liquid liquid extraction, the analyte is separated from the sample matrix using a chromatography stationary phase. The analyte sticks to the column and the water wash removes all species that do not adhere to the column. O Two solvents differe in their polarities are used where the analyte is extracted into the similar (close in polarity) solvent. The solid and liquid phase heterogenous mixture is centrefuged prior to sample measurement and analysis.Lets say I want to seperate compunds A,B through Column Chromotography. In the image attched 6 results of thin layer chromotography are shoen in different proportions of hexane to ethyl acetate. Which chromotography plate of the ones shown is the WORST system of dissolvlents or has the worst prpotion to seperate compounds A and B and explain why