E. coli strain BW25113 can grow on (D) Arg as the sole carbon source. A scientist  working with you on this project has discovered that mice encode a protease that can cleave between (D)Arg‐(D)Arg.  a. In a set of discrete steps outline how to construct this genomic library so that  recombinant expression can be performed in E. coli b. Once the genomic library has been constructed, describe in DISCRETE steps how  you would isolate the DNA sequence that encodes for the protease that cleaves  between (D)Ag-(D)Arg

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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E. coli strain BW25113 can grow on (D) Arg as the sole carbon source. A scientist 
working with you on this project has discovered that mice encode a protease that can
cleave between (D)Arg‐(D)Arg. 
a. In a set of discrete steps outline how to construct this genomic library so that 
recombinant expression can be performed in E. coli

b. Once the genomic library has been constructed, describe in DISCRETE steps how 
you would isolate the DNA sequence that encodes for the protease that cleaves 
between (D)Ag-(D)Arg

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