doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference as part of her literary research for her proposal on decreasing the amount of abnormal white blood cell growth associated with acute myeloid leukemia (AML) using a murine (mouse) model. She is being provided with 10 AML mice courtesy of her mentor. Can you help her out with her proposal? 1) Esther’s eventual goal is to design 20-26 bp RNA pieces that can bind to mRNA of AML and test it in a murine model. Of the choices below, which seems like the first step necessary to get her research off the ground? a) Make sure there is similarity between the murine AML gene and the human AML gene using a Clustal Omega alignment. b) BLAST the human version of the AML gene in NCBI. c) First, see if there is an existing mouse AML gene accession on NCBI to work with since that would negate the necessity to produce her own saving much time and money. d) Design the RNAi she will use for treatment
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A doctoral student in biology, Esther Mensah, is reviewing primary literature over RNAi interference as part of her literary research for her proposal on decreasing the amount of abnormal white blood cell growth associated with acute myeloid leukemia (AML) using a murine (mouse) model. She is being provided with 10 AML mice courtesy of her mentor. Can you help her out with her proposal?
1)
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