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Q: Identify and describe the 4 wet-heat methods that are used to control microbial growth.
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Q: DISCUSSION Discuss sterilization technique Autoclave advantages? Autoclave tape? Filtration
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Q: equipments can be used to determine turbidity in the sample tubes in testing of antimicrobial act
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Guide Questions:
- Differentiate the types of culture media based on its physical state and uses.
- What makes agar good support for microbial growth?
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- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.Guide Questions: Differentiate the types of culture media based on its physical state and uses. What makes agar good support for microbial growth? Define and contrast the terms “sterile” and “clean”. List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?Explain the significance of aseptic culture technique in growing microorganisms.
- Outline the different physical and chemical methods of sterilization. Briefly describe how each method control microbial growtha) Microbial products can be classified in three major categories which related to the microorganism growth curve. By giving examples of product based on the three major categories, sketch the microbial growth and product formation curves. Describe the phases involves in the growing of microorganism for the products formation. List all references with proper citations.Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:
- Identify and describe the 4 wet-heat methods that are used to control microbial growth.Name 2 indirect methods for measuring microbial growth and describe how each is performed. a. i. Advantage: ii. Disadvantage: b. i. Advantage: ii. Disadvantage:Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow
- Name the 5 main sources of external contamination associated with spent culture media during non-invasive PGT.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?Discuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages of each technique in a microbiology laboratory?