Determine the size of the DNA you would expect from the restriction digests of the GABRD and GABRD I284T PCRs. -Clearly write your null hypothesis for a chi-squared test of the viability of homozygous GABRD I284T variant. -Explain whether there is a difference in the levels of phosphorylated p44/42-MAPK antibodies and why you think that might be. Draw a diagram of a PCR cycle and give a hypothesis on how different GC content and different primer concentrations may effect the optimisation of a PCR. -Explain the process of agarose gel electrophoresis and how it is used to identify DNA fragments of different size, and what is the effect of using different agarose concentrations

Determine the size of the DNA you would expect from the restriction digests of the GABRD and GABRD I284T PCRs. -Clearly write your null hypothesis for a chi-squared test of the viability of homozygous GABRD I284T variant. -Explain whether there is a difference in the levels of phosphorylated p44/42-MAPK antibodies and why you think that might be. Draw a diagram of a PCR cycle and give a hypothesis on how different GC content and different primer concentrations may effect the optimisation of a PCR. -Explain the process of agarose gel electrophoresis and how it is used to identify DNA fragments of different size, and what is the effect of using different agarose concentrations

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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blaA ori lacl Plac rrgB rrgA lacZYA Recombinant Plasmid "B"
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