Describe how you would prepare a 500 ml of 0.35M Glycine pH2.2 from a 5M Glycine solution of pH 7. Show your calculations.
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Describe how you would prepare a 500 ml of 0.35M Glycine pH2.2 from a 5M Glycine solution of pH 7. Show your calculations.
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- You need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4. Please indicate the full solutions.What mass of sodium glycolate (NaC2H3O3) should be added to 400.0 mL of 1.00 M glycolic acid to produce a buffer solution with a pH of 4.00? Ka = 1.47 x 10-4
- TE buffer consists of 10 mM of Tris-Cl, pH 7.6 and 1mM of EDTA, pH 8.0. You need to prepare 3L of TE buffer and you have the following stock solutions: 500 mM Tris-Cl and 1 M EDTA. How will you prepare the 3L of TE? Write work clearlyAn aqueous antifreeze solution is 45.0% ethylene glycol () by mass. The density of the solution is 1.05. Calculate the molality, molarity, and mole fraction of the ethylene glycolPrepare 200 ml of a 0.125 M stock of NaCl from a 1 M concentrated stock solution of NaCl.
- You have just used up the last of a 1.2 % m/m solution of agarose in water. You require another 100mls. How many grams of Agarose do you require? Note 1ml of water=1g What is the % w/v of a 100ml solution containing 5g of NaCl?You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M KOH solution. If you have 730 mL of the acetic acid solution, how many milliliters of the KOH solution do you need to add to make a buffer of pH 5.43? The pKa of acetic acid is 4.76. Be sure to use appropriate significant figures.given a stock solution of 10% dextrose, how would you make 5 mL of a 0.9% dextrose solution? find mL of stock and mL of h20
- You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquidDetermine the pH for the following solutions. {H3O+}=2.4x10^-2 M80.00 mL of 0.350 M benzoic acid (K = 6.4×105) is titrated by 0.350 M NaOH. Calculate the pH of the acid solution before any titrant is added. PH Calculate the pH after 58.9 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic acid. pH = Calculate the pH after 80.00 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic ad pH = Calculate the pH after 110 mL of 0.350 M NaOH is added to 80.00 mL of 0.350 M benzoic acid. pH=