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- SEPARATION OF COMPONENTS BY CENTRIFUGATION Consider the following subcellular components and their corresponding sedimentation rates. Table 3. Sedimentation rates of four subcellular components for isolation. CENTRIFUGAL FORCE (xg) SUBCELLULAR COMPONENT CDCP 14896 AJRA 967 AGC 382 JCKSP 2378 Two centrifuge machines are available in your laboratory. Centrifuge A has a rotor diameter of 14 cm and a maximum speed of 3500 rpm while centrifuge B has a rotor diameter of 18 cm and a maximum speed of 14500 rpm. Construct a separation scheme of the four subcellular components considering the following parameters: The four components are separated into pellet/supernatant I and pellet/supernatant II. Only two centrifugation steps are performed; specify the centrifugal speeds (in rpm) per step. Show relevant calculations. Both centrifuge machines must be used in the procedure. Components of each fraction are indicated.Features of bacteria that aid _________ research aremonoploidy, which facilitates mutant identification,and rapid exponential growth, which allows recoveryof rare mutants.explanation on how bacteria mycobacterium ulcerans should have the ability to be isolated and grown in pure culture
- Prepare a dilution of phage from 1.5 x 1010 PFU/mL to 1.00 x 102 PFU/mL. Draw a dilution scheme to achieve this serial dilution considering the following: The volumes of each dilution should be 1.0mL. It is not advisable to perform any dilutions > 1/100. The first dilution in any titre determination of stock phage should use 10µl or less of stock.TOPIC: E. coli T4 (T4 Bacteriophage) Include a maximum of 3 sentences, a brief description of their growth. What are the structures responsible for growth and reproduction? What are their physical and nutritional (or chemical) requirements?What is the genus and species of your bacteria? And What led you to confirm this result and why? Please discuss. ☆My bacteria is Alcaligenes faecalis
- Σ P UTF-8"Chapter 12 Solutions 202 x 02-xythos.content.blackboardcdn.com/5b19550bob538/10621560?X-Blackboard-Expiration3=D1649052000 DWorksheet.docx Edits will not be automatically saved. aved changes MOu a Garamond 14. B. 三: 三 山 8. When placed in each of the following, indicate if a red blood cell will 1) not change 2) hemolyze 3) crenate 5%(m/v) glucose solution 1%(m/v) glucose solution 0.5%(m/v) NaCl solution 2%(m/v) NaCl solutionWhich molecules does E. coli not have to synthesize when grown in LB, compared to minimal media? Why would the presence of other (unknown) organisms and virusesconfuse studies on the growth a specific organism?Before development of a vaccine against this microbe, thedisease it caused accounted for two-thirds of bacterial meningi-tis cases during the first year of life but is still the number oneleading cause of mental retardation in patients who survive seri-ous disease due to permanent central nervous system disorders.What is the microorganism?(a) Haemophilus influenzae type B(b) Haemophilus influenzae type A(c) Neisseria meningitidis(d) Streptococcus pneumoniae(e) Listeria monocytogenes
- Why is E. coli O157:H7 an organism of concern in contaminated foods? The strain is represented as O157:H7. Which particular “parts” of the bacterial cell do the “O” and “H” refer to?O SC myEFSC с ← > C O Search the webesting 8MU376MpwkNtkrF5%252B26BVPP%252Bzg%253D%2. Question Image 1 3 6. 7. 8. with the figure? cracteristics ight differe dividuals can compared Q. Which of following can you do with the biotechnology depicted in the figure? • 0 1:47 Sign out || O|| | 11SEE MORE QUESTIONS