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What is the difference in composition and use between:
a. chemically defined media
b. complex culture media
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- Can you give me an example of a specific medium in culture media and fill the information below. General Information Specific a. Name of medium: b. Physical form: c. Category based on formulation: d. Purpose/uses/function: e. List of Ingredients and concentration (per 1000 ml) f. How to prepare?What type of medium is used to maintain and preserve specimens before clinical analysis? a. selective medium b. transport medium c. enriched medium d. differential mediumIllustrate the different solid tubed media formed 1. Deep/Butt solid tubed media2. Slant solid tubed media 3. Butt-slant solid tubed media
- Illustrate the step by step method of preparing culture media.List the two main types of culture media and how each differs and the types of microbes which grow in each type.Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?
- Mueller-Hinton agar needs to be made to specific standards in order to yield reliable, reproducible results. How deep is the agar on a Mueller-Hinton agar plate? a) 2 mm b) 4 mm c) 6 mm d) 8 mmWhat type of medium is best used to maintain a preserve specimen before clinical analysis? A. Enriched b. Selective c. Differential d. Reducing e. NutrientWhich of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
- Differentiate between defined and complex culture mediaHow can one be sure that the culture media are sterile or free from contaminationWhich specific example of a functional medium would you use to grow microbesfrom the following samples? Explain why it would be appropriate based on itsa. Air sample for bacteria:b. Sample from behind the ear for microbes:c. Sample from table top for fungi:
