Can you help me understand how to measure both protein A and B please
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- please explain the question, i am unable to input values of xPlease give numeric valueFigure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…
- How many angstrom would be in 5 μm? Question options: 50 500 5000 50000Give Detailed Solution with explanation needed (:don't give Handwritten answer))Use MS Excel to plot and determine the terms being asked: Please insert the excel file, thank you For each V (mmol/min), determine the value of [S] (mM) if Vmax = 5 mmol/min. Show your calculations. Using MS Excel, plot [S] (mM) versus V (mmol/min). Draw a line corresponding to the given Vmax parallel the x-axis and extend your plotted line to show its approach to Vmax. Provide your final plot [S] (mM) V (mmol/min) 10 1.2 1.7 2.1 2.2 2.5 Determine the values of Km and Vmax for the decarboxylation of β-keto acid given the following data. Please show and label your plot. Show your calculations and box your final [S] in mol/l V in mM/ min 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256
- Which of the phi/psi angles would be violating a ramachandran plot? Select all that apply: Phi= 90; Psi=-120 Phi= -120; Psi=120 Phi= 0; Psi=0 Phi= -90; Psi=-60 Phi= 120; Psi=-120Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
- Pls help me with the given question and explain your correct answer, thank you A group of absent-minded scientists has two numbers (25.4 and 2.54) written on a scrap of paper. One scientists says: “I remember that one of these values was the standard deviation and the other the standard error of the mean, but I can’t remember which is which”. a)Which number is the standard deviation, and which is the standard error of the mean? b)What was the sample size? SE= s/√(2√n)Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.