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- Determine the % inaccuracy by calculating the percent error of the micropipette at the set volume of 100 µL A. Standard deviation B. Covieffecrnt of varaiant C. % error (precision) Percent error = mean volume delivered -expected volume x 100 Expected volume RUN Weight Measurement (in g) 1 0.0985 2 0.0987 3 0.0985 4 0.0982 5 0.0984 6 0.0984 7 0.0981 8 0.0983 9 0.0982 10 0.0988 Total 0.9841 A. Standard deviation B. Covieffecrnt of varaiant C. % error (precision)When determining CFUs for a dilution of the same sample at 10-2 and 10-3 which of the following results would seem the most accurate to you? Explain why in the space below. A. 110,000 CFU/mL for 10-2; and 140,000 CFU/mL 10-3 B. 110,000 CFU/mL for 10-2; and 14,000 CFU/mL 10-3 C. 10,000 CFU/mL for 10-2; and 140,000 CFU/mL 10-3 D. none of the above8 Imipramine liquid contains 5mg/1mL. A patient requires 100mg twice daily. How many mL will they require per dose? Please give your answer as a whole number Answer: ml Show Calculator
- When 100 mg of a drug is administered as an IV bolus dose, the plasma concentrations (ug/mL) observed over time (hr) can be described by the following equation: C = 3.6e-0.105t C= 3-6e AUC 36 6 -0.105t 5. Calculate AUC(0-0) 0-105 = 34.28 6. What is the volume of distribution? Dose 100 VD= Co 3-6 7. Calculate the clearance of this drug. CI = KXVD CI = 0•105 x 27-8 %3D %3D Cl=2.9 8. What IV dose would be required to produce an initial plasma concentration of 10 ug/mL?1. compute for patient B result of his chloride analysis with the following data using mg/dL unit factor: 355/ mg/dL , Absorbance sample = 0.2148, Absorbance standard = 0.3017 a. 24.8 mg/L b. 25.27 mg/dL c. 2.7119 mmol/L d. 252.7 mg/dL 2. an adult patient suffering from debilitation is supected of calcium deficiency. His doctor requested for calcium anaylusis with the following data gathered standard known value is B: Absorabance is 0.2876. sample absorbance is 0.4291 compute for the calcium in mmol/L a. 53.6mmol/L b. 5.36 mmol/L c. 11.9 mmol/L d. 119mmol/L 3. calculate the value of sodium where the PREC solution has diclored. data gathered are the following: Absorbance of Reagent Blank = 0.3124 Absorbance of sample = 0.114, Absorbance of standard = 0.275 value is 150mmol/L a. 108.2 mmol/L b. 170.4 mmol/L c. 795.72 mmol/L d. 79.5 mmol/L1. Ifa Lineweaver-Burk plot gave a line with an equation of y=0.25 x +0.34, what are the values of KM and Vmax if the substrate concentration is in mM and the velocity in mM/s?
- The biexponential equation for a two compartment model drug is C1,t = 50.0e-1.64t + 20.0e-0.147t. The drug is administered as a rapid IV bolus at t = 0. After 2.00 hours how many mg/l will the drug concentration in the central compartment be.25. ATROVENT Nasal Spray contains 0.03% of ipratropium bromide in a 30-mL me- tered dose container. If the container is calibrated to deliver 345 sprays, calculate the volume of each spray, in microliters, and the medication content of each spray, in micrograms. 25. 86.96 µL /spray and 26.09 µg ipra- tropiumA. Kinetics Data 1/[S], mM- 0.5714 0.4608 0.3333 0.1818 0.0952 1/Velocity (min/mM) Inhibited 0.7246 0.5988 0.4694 0.3367 0.26109 Uninhibited 0.5154 0.4424 0.3508 0.2816 0.2277 B. Lineweaver-Burk Plot and Analysis Paste your Microsoft Excel Graph of the kinetic analysis. Compute for Vmax and Km values and identify the mode of enzyme inhibition involved.
- You have measured the following data for enzyme X. Substrate Vo concentration (micromolar (mM) Isec) 0.05 10 0.1 60 0.5 175 1 250 350 490 10 492 20 494 50 499 100 498 200 498A prescription order calls for procainamide 0.5 g. In the pharmacy, the technician uses a 1g/10 mL vial to prepare the dose, which is injected into a 150 mL D5W IV bag for infusion. Assuming conventional overfill for the IV bag, what should the flow rate be in mL/hr if the dose of the drug is 2 mg/min? Round to the nearest whole mL.BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.