Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant Brassica. Wildtype DNA 3’ CTT  AAT GCT CCG AAT CCA 5’ template strand 5’ GAA TTA CGA GGC TTA GGT 3’ non-template strand A new strain (Strain X) of Brassica is identified with the same region of the gene coding for protein Y: 3’ CTT  AAT GCT GCG AAT CCA 5’ template strand 5’ GAA TTA CGA CGC TTA GGT 3’ non-template strand   Compare the sequence of Wildtype with Strain X DNA, and note the following: Whether there is a mutation. If there is a mutation, what is the type of mutation (be as specific as possible) and explain the rationale for your decision.  Assuming this is the only difference between the Wildtype and Strain X, describe the potential impact of the mutation on the structure and function of the protein.

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant Brassica.

Wildtype DNA
3’ CTT  AAT GCT CCG AAT CCA 5’ template strand
5’ GAA TTA CGA GGC TTA GGT 3’ non-template strand

A new strain (Strain X) of Brassica is identified with the same region of the gene coding for protein Y:
3’ CTT  AAT GCT GCG AAT CCA 5’ template strand
5’ GAA TTA CGA CGC TTA GGT 3’ non-template strand

 

Compare the sequence of Wildtype with Strain X DNA, and note the following:

  1. Whether there is a mutation.
  2. If there is a mutation, what is the type of mutation (be as specific as possible) and explain the rationale for your decision. 
  3. Assuming this is the only difference between the Wildtype and Strain X, describe the potential impact of the mutation on the structure and function of the protein. 
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