Before using the API® 20E System, what test must be performed to confirm the identity of your unknown as a member of the family Enterobacteriaceae? Why is this important, and what is the expected result?
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Before using the API® 20E System, what test must be performed to confirm the identity of your unknown as a member of the family Enterobacteriaceae? Why is this important, and what is the expected result?
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- Identify a biochemical test that can differentiate Enterobacter agglomerans from Enterobacter cloacaeBefore using the EnteroPluri-Test System, what test must be performed to confirm the identity of your unknown as a member of the family Enterobacteriaceae? What is the expected result?An Enteropluri test (including VP), as used in Practical 4, gave the following results code: 7 2 O b. Lysine Oc. Lactose 3 O d. Glucose 5 This organism is capable of utilising which substrate to form a gas? This question is about interpretation of Enteropluri test data and not for identification (biocode may not exist). Oa. Arabinose 2
- EPKP O111: K58 is isolated from the feces of a sick child under 3 months of age with certain clinical manifestations in the bacteriological laboratory. 1. What diagnosis can be given to a child? 2.What method of microbiological diagnosis was used in this case? Describe its main stages. 3. Is it possible to use serodiagnosis to verification of coli-enteritis? What material is taken from the patient for this purpose and what is used: glass-agglutination test, direct agglutination test or IHAT?A light yellow colony from a skin lesion grew aerobically and tested as catalase positive and coagulase negative. The organism stained as GPC. The organism was modified oxidase positive, bacitracin (0.04U) susceptible, and resistant to lyphostatin. What is the identification of the organism? Explain the lyphostatin test.What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?
- What is the genus and species of your bacteria? And What led you to confirm this result and why? Please discuss. ☆My bacteria is Alcaligenes faecalisWhat is the difference between the total and faecal coliform MPN assay?Consider the photos here which demonstrate antibiotic sensitivities of Staphylococcus aureus strains as determined by the Kirby-Bauer method. Abbreviations are as follows: C = chloramphenicol; CC = clindamycin; CZ = cefazolin; E = erythromycin; NOR = norfloxacin; OX = oxacillin; P = penicillin; RA = rifampin; SAM = sulbactam-ampicillin; SXT = sulfatrimethoprim; TE = tetracycline; VA = vancomycin. Imagine that only two cellular changes occurred in the original strain (the first image, on the top) that resulted in the resistance pattern of the strain in the second image (on the bottom). Which combination of mechanisms could explainthese results?Choose one or more: A.expression of efflux pumps B.overproduction of PABA C.production of β-lactamase D.altered penicillin-binding protein E.modification of either 50S or 30S ribosomal subunits F.altered DNA gyrase
- Results of Kirby Bauer test on E. Coli with penicilin , ampicilin, tetracycline, chloramphenicol disks showed zero zone of inhibition while there were zones around all these disks on S. aureus this should be interpreted as this drug is narrow spectrum a) True b) FalseNitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You inoculated your Nitrate broth with your unknown; after incubation, you added 5 drops of reagent A and 5 drops of reagent B. You observe a red color within 2 minutes. Is this a + or a - reaction? Assuming you did not get a red color, you then added a pinch of Zinc and this time tou obtained a red color. What does this mean?Explain how PEMBA (polymyxin-pyruvate-egg yolk-mannitol-bromthymol blue-agar) is used to isolate, differentiate and enumerate Bacillus cereus from food sample.