b. Please draw the structure of the polypeptide: threonine-lysine-glutamine-valine at pH 7.0, and identify the alpha carbons. C. Estimate the isoelectric point of the polypeptide in (b.)?
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- a. Draw the peptide chain (indicate if it is solid or hatched wedges) for the following: Glycine-(L)-Arginine-(D)-Proline-(L)-Glutamic Acid b. Compute for the isoelectric point. Show solution.A. Write the structure of the following peptide at pH 5.0 and calculate its net charge at this pH. Asp-His-Tyr-Arg-Lys-Leu-Thr-Gln. Based on the pKa value of the ionizable groups. B. A polypeptide consisting only of L-glutamate residues (poly-L-glutamate) may have a random coil or helical structure depending on pH. Explain this behavior by indicating at what pH values the helical structure will be favored.a. Use three-letter amino acid abbreviations to write the representation for the tripeptide shown here. i H₂N-CH-C-N-CH-C-N-CH-C-0- CH₂ H -NH H ī CH₂ SH -CH-C-0- CH-OH CH3 b. Give the full name of the amino acid at the C-terminal end of this tripeptide. H₂N-CH-C-N-CH-C-N-CH-C-O- -CH-C-N-CH-C CH₂ c-o- CH₂ OH
- Peptide A: AFEQHSR Calculate the isoelectric point of Peptide A using the pKa values in Table 4-1 of Voet, Voet and Pratt. Assume that the pKa values for the N-terminal amino group and C- terminal carboxyl groups are 8.0 and 3.5, as previously discussed in class. 3.785 5.055 7.02 10.24 3.50A/B: 3. Amino acid residues and peptide bonds (a) Draw the chemical structure of F, S and H joined by peptide bonds and also connected to neighboring residues as found in a protein. (b) Include all charges as expected at physiological pH (pH = 7.5) () Rank these three amino acid residues by hydrophobicity, pKa, and by nucleophilicity (d) For the central amino acid residue, identify the phi and psi dihedral angles by drawing a curved arrow indicating rotation about the chemical bond. (e) What group(s) eclipse each other if Phi = 0 degrees? Words and Pictures. (f) While Phi = 0 is not well tolerated, Psi = 0 is not uncommon, a so-called “allowed" but not most-favored region of the Ramachandran plot. What group(s) eclipse each other if Psi = 0 degrees and why is this less problematic than the situation created by Phi = 0. Words and Pictures H₂N* Pka: 9.13 H₂C с C._hydrophobicity: 1. phenylalanine (most) 2. Serine 3. Histidinelleast) ده H H. HIN OH I CH₂ CHO pka: 1. Serine (nighest) 2.…A. Where would you expect valine, leucine, and isoleucine to be located in a polypeptide? (Inside or outside)? Explain your answer using the properties of the amino acids listed. B.How would your answer change, if the amino acids were Lysine and Histidine?
- Two peptide sequences are shown below.Peptide A: N-term – GDL – C-termPeptide B: N-term – GTL – C-terma. Draw each peptide at physiological pH. Circle each ionizable functional group in your drawing.b. Briefly explain why changing aspartate to threonine changes the number of ionizable functionalgroups in the peptide chain.c. Briefly explain why the functional group found in aspartate’s side chain is ionizable but the functionalgroup found in threonine’s side chain is not.a. what general category would you place this molecule in of the four categories of biomolecules? b. List 5 functional groups you see in this molecule?a. Suppose that you have the peptide Ala-Gly-Tyr-His-Leu and you treat it with FDNB and then 6M HCl. Draw the structures of all the products that you will have in solution (assume all reactions to go to completion).
- *H3N -Č-H CH2 он Tyrosine a. Draw the structures of tyrosine as different pH values. Please refer to the lecture notes for the pKa values b. Identify the structures that exist at pH 2 c. Solve for the plFor the following pentapeptides: Ser-Glu-Gly-His-Ala and Gly-His-Ala-Glu-Ser A. Compute their isoelectric pH (pI). Show full solution. Use standard pKa values. B. Do these peptides with the same amino acid composition have different net charges at pH 7.0? Explain briefly. C. Would you expect the titration curves of the two peptides to differ? Why or Why not?Classify the fatty acid with the following structural formula in the ways indicated. COOH a. What is the type designation (SFA, MUFA, or PUFA) for this fatty acid? b. On the basis of carbon chain length and degree of unsaturation, what is the numerical shorthand designation for this fatty acid? c. To which "omega" family of fatty acids does this fatty acid belong? d. What is the “delta" designation for the carbon chain double-bond location for this fatty acid?