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- Under normal physiological conditions, aspartic acid will be favored over aspartate. True FalseWhich of the following is false about chymotrypsin? A Hydrolytic cleavage of a peptide bond by chymotrypsin involves two nucleophilic attacks to the substrate B It is activated in the presence of trypsin C It is synthesized in the thyroid gland D Polypeptide chains in chymotrypsin are linked by S-S bondsExplain why triglycerides have a glucose-sparing effect.
- Explain why adipocytes need glucose as well as fatty acids in order to synthesize triacylglycerols.You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%In 2-3 paragraphs (must be typed) explain: Discuss the regulation of cholesterol synthesis.
- Process of exocytosis of insulinin triacylglycerol mobilization, triacylglycerol molecules is activated by: phosphorylation hydrolysis reduction oxidationThe “keto diet” is a dietary regimen in which carbohydrates are severely restricted, protein intake is low, and fat comprises 70–90% of daily calories. The diet causes “nutritional ketosis,” which is a different metabolic state than the ketosis that occurs in an untreated diabetic. The normal ketone body concentration is less than 0.6 mM. Explain why an individual on the keto diet may have a blood ketone body concentration of up to 3 mM. What biochemical pathways are active in an individual adhering to the keto diet? What pathways are largely inactive? Protein intake is kept low because a high-protein diet would inhibit ketosis. Explain why. Why might the keto diet lead to weight loss?
- The active form of insulin contains No disulfide bonds Intra-chain disulfide bonds Inter-chain disulfide bonds Both inter- and intra-chain disulfide bonds.Explain Specificity of phospholipases.Provide a diagram of the EPH RECEPTOR B2 (EPHB2) structure. Give annotations of binding sites and enzyme active sites.