Assuming the use of the same stationary and mobile phases, how would the Rf of a compound change ifI had used a TLC plate that was twice as long as the plate used in my experiment?
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Assuming the use of the same stationary and mobile phases, how would the Rf of a compound change if
I had used a TLC plate that was twice as long as the plate used in my experiment?
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- A particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?(iii) What would be the effect on the retention time and order of eluting if the C18 column is substituted with a -CN column?When stating the Rf value of a compound, why is it also necessary to state the mobile phase that was used?
- While performing absorbance measurements of standard solutions, Taranpreet used - 8 mL of solution in the cuvette for each determination. She then repeated the same measurements using - 6 mL of solution In the cuvette for each determination, For each set of measurements, she used the same spectrometer with the same cuvette, and assume that for each set of measurements the cuvette was filled high enough such that the light from the spectrometer passed through the solution. Which statement is true? O a) Using a lower volume in the cuvette will have no effect on the measured values O b) Using a lower volume in the cuvette will result in a smaller slope for the calibration curve c) Using a lower volume in the cuvette will result in a lower concentration of analyte determined in the unknown sample O d) Using a higher volume in the cuvette may result in absorbance values that are above the detection range of the spectrometer O e) Using a higher volume in the cuvette may result in a…Given the background IR spectra, rank the three methods (Nujol mull, CCl4 solution, and KBr pellet) in order of best to worst spectra collected on a solid sample and explain your ranking.As1...If a TLC plate shows only one spot with an Rf value of 0.98, does this mean the sample is pure? Briefly explain why or why not.
- What characteristic makes a photodiode array spectrophotometer suitable for measuring the spectrum of a compound as it emerges from a chromatography column and a dispersive spectrophotometer not suitable? What is the disadvantage of the photodiode array spectrophotometer?Why are instrumental methods (e.g. UV Vis spectroscopy) considered as comparative methods?The concentration of Na* in the sea water sample was determined with atomic fluorescence spectroscopy (AFS). 35.00 mL of the sea water sample produce an AFS signal of 0.425. Then, 5.00 mL of 0.00215 M Nat standard were added to another 35.00 mL of the sea water sample, and this spiked sample gives an AFS signal of 0.724. What would be the concentration of Na* in the sea water sample? 0.0268 M 0.00753 M 0.0109 M 0.587 M 0.00324 M
- 1. Describe the different types of analytes which can be analyzed using the different methods of optical analyses. 2. Why is it that absorbance readings be done at lambda max?What are the parameters that can be used for analytes identification and quantification using Cyclic Voltammetry, respectively?If you were to try a column one-half the length of the one you usually used, how do you think the retention times of the butenes would be affected? How do you think the separation of the peaks would be affected? How do you think the width of each peak would be affected?
