An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?
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An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?
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- An amino acid mixture consisting of lysine,leucine, and glutamic acid is to be separated by ion-exchangechromatography, using a cation-exchange resin at pH 3.5, with theeluting buffer at the same pH. Which of these amino acids will beeluted from the column first? Will any other treatment be neededto elute one of these amino acids from the column?An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
- Define the following:- pH- Buffer- pKaA glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equationUsing DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest experimental pH range used to seperate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: Ending pH:
- If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.Will the given lipid test positive in the Ninhydrin test?Cyclophosphamide, after reconstitution, has a concentration of 20 mg/mL. The patient, who weighs 67 kg and is 155 cm tall, is ordered cyclophosphamide in 250 mL NS. How many mL will be added to the bag?
- Give the complete solution.A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?